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M71
Kligler Iron Agar
Polypeptone peptone | 20 g |
Lactose | 20 g |
Dextrose | 1 g |
NaCl | 5 g |
Ferric ammonium citrate | 0.5 g |
Sodium thiosulfate | 0.5 g |
Agar | 15 g |
Phenol red | 0.025 g |
Distilled water | 1 liter |
Heat with agitation to dissolve. Dispense into 13 x 100 mm screw-cap tubes and autoclave 15 min at 121°C. Cool and slant to form deep butts. Final pH, 7.4 ± 0.2.
Commercially available from Difco, BBL, and Oxoid.
For use with halophilic Vibrio spp., add NaCl to a final concentration of 2-3%.
Hypertext updated by rim/cjm 2005-OCT-21