M70
King's O/F Basal Medium
|
M70
King's O/F Basal Medium
| Trypticase (or casitone) | 2 g |
| Phenol red, 1.5% solution | 2 ml |
| Agar | 3 g |
| Distilled water | 1 liter |
Heat with agitation to dissolve agar. Adjust pH to 7.3 ± 0.2. Dispense 100 ml portions to flasks. Autoclave 15 min at 121°C. Cool to 50°C.
Dissolve 10 g quantities of carbohydrates in 100 ml distilled water. Sterilize by filtration through 0.22 µm membrane. Add 10 ml concentrate to 90 ml melted base and mix. Aseptically dispense 3 ml portions to sterile 13 x 100 mm tubes.
Hypertext updated by rim/cjm 2005-OCT-21
