M62
HC Agar
(for "Hemorrhagic Colitis" E. coli strains)
|
M62
HC Agar
(for "Hemorrhagic Colitis" E. coli strains)
| Tryptone | 20.0 g |
| Bile salts No. 3 | 1.12 g |
| NaCl | 5.0 g |
| Sorbitol | 20.0 g |
| MUG reagent | 0.1 g |
| Bromcresol purple | 0.015 g |
| Agar | 15.0 g |
| Distilled water (deionized) | 1 liter |
Dissolve ingredients in distilled water by heating with stirring. Autoclave 15 min at 121°C. Final pH, 7.2 ± 0.2.
NOTE: The MUG reagent is not essential for the enzyme-labeled monoclonal antibody procedure.
For DNA probe: If colonies are not to be isolated by growth and metabolic characteristics, MUG reagent may be omitted. Plates may be kept 3-4 weeks if wrapped and stored at 4°C. Colonies do not attach well on filters if plates are too dry.
If HC agar is to be utilized fully, see Chapter 24, ref. 105.
MUG reagent may be purchased from HACH Company, P.O. Box 389, Loveland, CO.
| Storage: | 3-4 wk at 4°C if plates wrapped. |
Hypertext updated by rim/cjm 2005-OCT-21
