|
M61
Hektoen Enteric (HE) Agar
Caution!! | |
Storage | 1 day only |
Peptone | 12 g | NaCl | 5 g |
Yeast extract | 3 g | Sodium thiosulfate | 5 g |
Bile salts No. 3 | 9 g | Ferric ammonium citrate | 1.5 g |
Lactose | 12 g | Bromthymol blue | 0.065 g |
Sucrose | 12 g | Acid fuchsin | 0.1 g |
Salicin | 2 g | Agar | 14.0 g |
Distilled water | 1 liter |
Heat to boiling with frequent agitation to dissolve. Boil no longer than 1 min. Do not overheat. Cool in water bath. Pour 20 ml portions into sterile 15 x 100 mm petri dishes. Let dry 2 h with lids partially removed. Final pH, 7.5 ± 0.2. Do not store more than 1 day.
Hypertext updated by rim/cjm 2005-OCT-21