U.S. Food & Drug Administration
Center for Food Safety & Applied Nutrition

Bacteriological Analytical Manual Online
January 2001

M58
Ham's F-10 Medium

(commercial preparation is preferred)

(Return to Table of Contents)


L-Alanine 8.91 mg Sodium pyruvate 110 mg
L-Arginine Hcl 211.00 mg Thymidine 0.727 mg
L-Asparagine-H2O 15.00 mg Biotin 0.024 mg
L-Aspartic acid 13.30 mg Choline chloride 0.698 mg
L-Cysteine·2HCl 35.12 mg Folic acid 1.320 mg
L-Glutamine 146.20 mg Isoinositol 0.541 mg
L-Glutamic acid 14.70 mg Niacinamide 0.615 mg
Glycine 7.51 mg D-Calcium pantothenate

0.715 mg
L-Histidine HCl·H2O 21.00 mg Pyridoxine HCl

0.206 mg
L-Isoleucine 2.60 mg Riboflavin 0.376 mg
L-Leucine 13.10 mg Thiamine HCl 1.010 mg
L-Lysine HCl 29.30 mg Vitamin B12 1.360 mg
L-Methionine 4.48 mg CaCl2·2H2O 44.10 mg
D-Phenylalanine 4.96 mg CuSO4·5H2O 0.0025 mg
L-Proline 11.50 mg FeSO4·7H2O 0.83 mg
L-Serine 10.50 mg KCl 285.00 mg
L-Threonine 3.57 mg KH2PO4 83.00 mg
L-Tryptophan 0.60 mg MgSO4·7H2O 152.80 mg
L-Tyrosine 1.81 mg NaCl 7400.00 mg
L-Valine 3.50 mg NaHCO3 1200.00 mg
Glucose 1100.0 mg NaH2PO4·H2O 290.0 mg
Hypoxanthine 4.08 mg ZnSO4·7H2O 0.028 mg
Lipoic acid 0.2 mg Phenol red 1.2 mg
Distilled water 1 liter

 

 

 

 

 

 

 

Dissolve ingredients in water. Sterilize by filtration. Final pH, 7.0 ± 0.2. Check sterility before use.



Hypertext Source: Bacteriological Analytical Manual, 8th Edition, Revision A, 1998.

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Hypertext updated by rim/cjm 2005-OCT-21