U.S. Food & Drug Administration
Center for Food Safety & Applied Nutrition

Bacteriological Analytical Manual Online
January 2001

M46
Eagle's Minimal Essential Medium (MEME)
(with Earle's salts and nonessential amino acids)

(Return to Table of Contents)


L-Alanine 8.9 mg L-Valine 46.0 mg
L-Arginine HCl 126.0 mg L-Tyrosine (disodium salt) 52.1 mg
L-Asparagine-H2O 150.0 mg D-Calcium pantothenate 1.0 mg
L-Aspartic acid 13.3 mg Choline chloride 1.0 mg
L-Cystine.2HCl 31.29 mg Folic acid 1.0 mg
L-Glutamic acid 14.7 mg Isoinositol 2.0 mg
L-Glutamine 292.0 mg Nicotinamide 1.0 mg
L-Glycine 7.5 mg Pyridoxal HCl 1.0 mg
L-Histidine HCl.H2O 42.0 mg Riboflavin 0.1 mg
L-Isoleucine 52.0 mg Thiamine HCl 1.0 mg
L-Leucine 52.0 mg Glucose 1000.0 mg
L-Lysine HCl 72.5 mg CaCl2.2H2O 265.0 mg
L-Methionine 15.0 mg KCl 400.0 mg
D-Phenylalanine 32.0 mg MgSO4.7H2O 200.0 mg
L-Proline 11.5 mg NaCl 6800.0 mg
L-Serine 10.5 mg NaHCO3 2200.0 mg
L-Threonine 48.0 mg NaH2PO4.H2O 140.0 mg
L-Tryptophan 10.0 mg Phenol red 10.0 mg
Distilled water 1 liter

 

 

 

 

 

 

Dissolve ingredients in distilled water. Sterilize by filtration. Final pH, 7.2 ± 0.2.

 


 


Hypertext Source: Bacteriological Analytical Manual, 8th Edition, Revision A, 1998.

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Hypertext updated by rim/cjm 2005-OCT-21