U.S. Food & Drug Administration
Center for Food Safety & Applied Nutrition

Bacteriological Analytical Manual Online
January 2001

M40
Christensen's Urea Agar

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Base

Peptone 1 g
NaCl 5 g
Dextrose 1 g
KH2PO4 2 g
Phenol red
(6 ml of 1:500 solution)		 0.012 g
Agar 15 g
Distilled water 900 ml

 

Dissolve all ingredients except urea in 900 ml water (basal medium).

For halophilic Vibrio spp., add extra 15 g NaCl (final NaCl concentration, 2%).

Autoclave 15 min at 121°C. Cool to 50-55°C.



Urea concentrate

Urea 20 g
Distilled water 100 ml

Dissolve urea in 100 ml water.



Filter-sterilize; add aseptically to cooled basal medium. Mix. Final pH, 6.8 ± 0.1. Dispense to sterile tubes or petri dishes. Slant tubes for 2 cm butt and 3 cm slant.


Hypertext Source: Bacteriological Analytical Manual, 8th Edition, Revision A, 1998.

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Hypertext updated by rim/cjm 2005-OCT-21