U.S. Food & Drug Administration
Center for Food Safety & Applied Nutrition

Bacteriological Analytical Manual Online
January 2001

M37
Cetrimide Agar

(Return to Table of Contents)


Pancreatic digest of gelatin 20.0 g
Magnesium chloride 1.4 g
Potassium sulfate 10.0 g
Agar 13.6 g
Cetrimide
(cetyl trimethyl ammonium bromide)		 0.3 g

Suspend 45.3 g of ingredients or commercial powder (PseudoselTM, BBL; Becton-Dickinson) in 1 liter of purified water. Add 10 ml glycerol. Mix well. Heat to boiling, agitating frequently; maintain boiling for 1 min to dissolve powder.

Autoclave at 118°C for 15 min.

 

Final pH, 7.2 ± 0.2. Bacto cetrimide agar base plus glycerol (Difco) is a similar medium.



Hypertext Source: Bacteriological Analytical Manual, 8th Edition, Revision A, 1998.

Home   |   B A M   |   B A M Media  |   Bad Bug Book

Hypertext updated by rim/cjm 2005-OCT-21