|
M35
Cefsulodin-Irgasan Novobiocin
(CIN) Agar
or Yersinia Selective Agar
(YSA)
Special peptone | 20 g |
Yeast extract | 2 g |
Mannitol | 20 g |
Pyruvic acid (Na salt) | 2 g |
NaCl | 1 g |
MgSO4.7H2O (10 mg/ml) | 1 ml |
Agar | 12 g |
Distilled water | 756 ml |
Irgasan 0.40% in 95% ethanol | 1 ml |
Sodium desoxycholate | 0.5 g |
Distilled water | 200 ml |
Bring to boil with stirring; cool to 50-55°C.
May be stored at -70°C. Thaw to room temperature just before use.
Preparation: Add ingredients for solution A (basal medium) to water and bring to boil with stirring. Cool to about 80°C (10 min in 50°C water bath). Add solution B (Irgasan) and mix well. Cool to 50-55°C. Add solution C (desoxycholate); solution should remain clear. Add solutions D through H. Slowly add solution I with stirring. Adjust pH to 7.4 with 5 N NaOH. Dispense 15-20 ml into each petri dish. Commercially prepared dehydrated Yersinia selective agar (Difco) with supplements may be substituted. Follow manufacturer's instructions for preparation.
Hypertext updated by rim/cjm 2005-OCT-21