U.S. Food & Drug Administration
Center for Food Safety & Applied Nutrition

Bacteriological Analytical Manual Online
January 2001

M23
Brain Heart Infusion (BHI) Agar (0.7%)
(for staphylococcal enterotoxin)

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Prepare a suitable quantity of brain heart infusion broth (M24). Adjust pH to 5.3 with 1 N HCl. Add agar to give 0.7% concentration. Dissolve by minimal boiling. Dispense 25 ml portions into 25 x 200 mm test tubes. Autoclave 10 min at 121°C.


Hypertext Source: Bacteriological Analytical Manual, 8th Edition, Revision A, 1998.

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