U.S. Food & Drug Administration
Center for Food Safety & Applied Nutrition

Bacteriological Analytical Manual Online
January 2001

M22
Blood Agar Base #2
(Difco)

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Proteose peptone 15 g
Liver digest 2.5 g
Yeast extract 5 g
NaCl 5 g
Agar 12 g
Distilled Water 1 liter

 

Autoclave at 121°C for 15 min. For blood agar, reduce water to 950 ml. Add 50 ml defibrinated (whole or lysed) horse blood and FBP (4 ml to agar + blood) after autoclaving and cooling to 48°C. Final pH, 7.4 ± 0.2.

 



Hypertext Source: Bacteriological Analytical Manual, 8th Edition, Revision A, 1998.

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