|
M20a
Blood Agar Base
(commercially available blood agar base may be used)
Infusion from beef heart | 500 g |
Tryptose | 10 g |
NaCl | 5 g |
Agar | 15 g |
Sheep blood (sterile, defibrinated) |
50 ml |
Distilled water | 1 liter |
Suspend ingredients except blood to dissolve. Autoclave 15 min at 121°C. Cool to 45-50°C. Add 50 ml blood (room temperature), and mix. Dispense into sterile petri dishes. Final pH, 6.8 ± 0.2.
Hypertext updated by rim/ear/cjm 2005-SEP-29