|
M2
Acetamide Medium
| KH2PO4 | 0.5 M | 14 ml |
| K2HPO4 | 0.5 M | 6 ml |
| Agar | 0.5 g | |
| Distilled water | 400 ml | |
Heat with agitation to dissolve agar. Add 1 ml PR-CV (500X concentrate).
| Acetamide | 1 g |
| Distilled water | 100 ml |
Store over CHCl3 in screw-cap container. Stable indefinitely at room temperature.
| Phenol red | 2 g |
| Crystal violet | 0.2 g |
| Distilled water | 200 ml |
Add 5 N NaOH until ingredients are dissolved.
Add 0.8 ml basal medium to 13 x 100 mm tube. Add 0.2 ml acetamide solution. Steam (100°C) 10 min. Cool.
Hypertext updated by rim/ear/cjm 2005-OCT-21