Mix ingredients, steam to dissolve agar and sterilize for 15 min at 121°C. Temper medium to 45°C and pour plates under aseptic conditions.

To prepare slants dispense 5-6 ml of steamed medium (before autoclaving) into each of several 16 x 125 mm screw-cap tubes, loosely cap tubes and sterilize as above. After autoclaving lay tubes in a slanting position and let them cool.

This medium is recommended as a general maintenance medium.

Hypertext updated by rim/cjm 2005-OCT-21