|
M174
Violet Red Bile Agar (VRBA)
Yeast extract | 3.0 g |
Peptone or gelysate | 7.0 g |
NaCl | 5.0 g |
Bile salts or bile salts No. 3 | 1.5 g |
Lactose | 10.0 g |
Neutral red | 0.03 g |
Crystal violet | 0.002 g |
Agar | 15.0 g |
Distilled water | 1.0 liter |
 
 
Suspend ingredients in distilled water and let stand for a few min. Mix thoroughly and adjust to pH 7.4 ± 0.2. Heat with agitation and boil for 2 min. Do not sterilize. Before use, cool to 45°C and use as a plating medium. After solidification, add a cover layer above the agar of approximately 3.0 to 4.0 ml to prevent surface growth and spreading of colonies.
Hypertext updated by rim/cjm 2005-OCT-21