U.S. Food & Drug Administration
C enter for Food Safety & Applied Nutrition

B acteriological A nalytical M anual Online
January 2001; Updated February 2008

M171
Urea Broth

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Urea 20 g
Yeast extract 0.1 g
Na2HPO4 9.5 g
KH2PO4 9.1 g
Phenol red 0.01 g
Distilled water 1 liter

 

Dissolve ingredients in distilled water. DO NOT HEAT. Sterilize by filtration through 0.45 µm membrane. Aseptically dispense 1.5-3.0 ml portions to 13 x 100 mm sterile test tubes. Final pH, 6.8 ± 0.2.

 

 



Hypertext Source: Bacteriological Analytical Manual, 8th Edition, Revision A, 1998.

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