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M166
Tryptose Blood Agar Base
Tryptose | 10 g |
Beef extract | 3 g |
NaCl | 5 g |
Agar | 15 g |
Distilled water | 1 liter |
Suspend ingredients in distilled water, mix thoroughly, and heat with occasional
agitation. Boil about 1 min. Fill 16 x 150 mm tubes 1/3 full and cap or plug to maintain
aerobic conditions. Autoclave 15 min at 121°C. Before media solidify, incline tubes to
obtain 4-5 cm slant and 2-3 cm butt.
Hypertext updated by rim/cjm 2005-OCT-21