U.S. Food & Drug Administration
Center for Food Safety & Applied Nutrition

Bacteriological Analytical Manual Online
January 2001

M166
Tryptose Blood Agar Base

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Tryptose 10 g
Beef extract 3 g
NaCl 5 g
Agar 15 g
Distilled water 1 liter


Suspend ingredients in distilled water, mix thoroughly, and heat with occasional agitation. Boil about 1 min. Fill 16 x 150 mm tubes 1/3 full and cap or plug to maintain aerobic conditions. Autoclave 15 min at 121°C. Before media solidify, incline tubes to obtain 4-5 cm slant and 2-3 cm butt.



Hypertext Source: Bacteriological Analytical Manual, 8th Edition, Revision A, 1998.

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