U.S. Food & Drug Administration
Center for Food Safety & Applied Nutrition

Bacteriological Analytical Manual Online
January 2001

M164
Tryptone (Tryptophane) Broth, 1%

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Tryptone or trypticase 10 g
Distilled water 1 liter

Dissolve and dispense 5 ml portions into 16 x 125 or 16 x 150 mm test tubes. Autoclave 15 min at 121°C. Final pH, 6.9 ± 0.2.



Hypertext Source: Bacteriological Analytical Manual, 8th Edition, Revision A, 1998.

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