U.S. Food & Drug Administration
Center for Food Safety & Applied Nutrition

Bacteriological Analytical Manual Online
January 2001

M151
Trypticase-Peptone-Glucose-Yeast Extract Broth
(TPGY)

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Trypticase 50 g
Peptone 5 g
Yeast extract 20 g
Dextrose 4 g
Sodium thioglycollate 1 g
Distilled water l liter

 

 

Dissolve solid ingredients and dispense 15 ml in 20 x 150 mm tubes. Autoclave tubes 10 min at 121°C. Final pH, 7.0 ± 0.2. Refrigerate at 5°C.

 



Hypertext Source: Bacteriological Analytical Manual, 8th Edition, Revision A, 1998.

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