M150
Trypticase Novobiocin (TN) Broth
|
M150
Trypticase Novobiocin (TN) Broth
| Trypticase soy broth | 30 g |
| Bile salts No. 3 | 1.5 g |
| Dipotassium phosphate | 1.5 g |
| Novobiocin | 20 mg |
| Distilled water | 1 liter |
Dissolve all ingredients except novobiocin by heating and stirring; autoclave at 121°C for 15 min. Prepare stock solution of novobiocin by adding 20 mg monosodium novobiocin per ml of distilled water. Filter-sterilize.
Make fresh stock each time of use, or store frozen at -10°C in the dark (compound is light-sensitive) for not more than 1 month (half-life is several months at 4°C). Add 1 ml stock solution per liter of medium.
| Make up fresh or |
|
| Storage: | Frozen [-10°C] dark conditions up to 1 month |
Hypertext updated by rim/cjm 2005-OCT-21
