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M144
T1N1 Medium
Trypticase | 10 g |
NaCl | 10 g |
Agar (if solid medium is preferred) | 20 g |
Distilled water | 1 liter |
Heat if necessary to dissolve ingredients. Dispense into 16 x 125 mm screw-cap tubes (if tubed medium is required). Autoclave 15 min at 121°C. Slant tubes until cool or let medium cool to 50 °C and pour into 15 x 100 mm petri dishes. Final pH, 7.1 ± 0.2.
Hypertext updated by rim/cjm 2005-OCT-21