U.S. Food & Drug Administration
Center for Food Safety & Applied Nutrition

Bacteriological Analytical Manual Online
January 2001

M140
Sporulation Broth
(for Clostridium perfringens)

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Polypeptone 15 g
Yeast extract 3 g
Starch, soluble 3 g
MgSO4 (anhydrous) 0.1 g
Sodium thioglycollate 1 g
Na2HPO4 11 g
Distilled water 1 liter

 

 

Adjust pH to 7.8 ± 0.1. Dispense 15 ml portions into 20 x 150 mm screw-cap tubes. Autoclave 15 min at 121°C.



Hypertext Source: Bacteriological Analytical Manual, 8th Edition, Revision A, 1998.

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