U.S. Food & Drug Administration
Center for Food Safety & Applied Nutrition

Bacteriological Analytical Manual Online
January 2001

M135
Sheep Blood Agar

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Blood agar base (Oxoid No. 2) 95 ml
Sterile sheep blood, defibrinated 5 ml

Rehydrate and sterilize base as recommended by manufacturer. Agar and blood should both be at 45-46°C before blood is added and plates are poured.


Commercial pre-poured sheep blood agar plates may be used.



Hypertext Source: Bacteriological Analytical Manual, 8th Edition, Revision A, 1998.

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