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M133
Sabouraud's Dextrose Broth and Agar
Polypeptone or neopeptone | 10 g |
Dextrose | 40 g |
Distilled water | 1 liter |
Dissolve completely and dispense 40 ml portions into screw-cap bottles. Final pH, 5.8. Autoclave 15 min at 118-121°C. Do not exceed 121°C.
For Sabouraud's dextrose agar, prepare broth as above and add 15-20 g agar, depending on gel strength desired. Final pH, 5.6 ± 0.2. Dispense into tubes for slants and bottles or flasks for pouring plates. Autoclave 15 min at 118-121 °C.
Hypertext updated by rim/cjm 2005-OCT-21