U.S. Food & Drug Administration
Center for Food Safety & Applied Nutrition

Bacteriological Analytical Manual Online
January 2001

M128
Pseudomonas Agar F
(for fluorescein production)

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Use Difco product, if available.

Proteose peptone No. 3 (Difco) 20.0 g
Tryptone (Difco) 10.0 g
Dipotassium phosphate 1.5 g
Magnesium sulfate 0.73 g
Glycerol (Difco) 10.0 g
Agar 15.0 g
Distilled water 1 liter

 

 

Add medium powder and glycerol to water; mix. Heat to boiling to dissolve ingredients. Autoclave at 121°C for 15 min. Final pH, 7.0.



Hypertext Source: Bacteriological Analytical Manual, 8th Edition, Revision A, 1998.

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