U.S. Food & Drug Administration
Center for Food Safety & Applied Nutrition

Bacteriological Analytical Manual Online
January 2001

M120
Peptone Sorbitol Bile Broth

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Na2HPO4 8.23 g
NaH2PO4 · H2O 1.2 g
Bile salts No. 3 1.5 g
NaCl 5 g
Sorbitol 10 g
Peptone 5 g
Distilled water 1 liter

 

 

 

Dispense 100 ml into Wheaton bottles. Autoclave 15 min at 121°C. Final pH, 7.6 ± 0.2.



Hypertext Source: Bacteriological Analytical Manual, 8th Edition, Revision A, 1998.

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Hypertext updated by rim/cjm 2005-OCT-21