M12
Anaerobic Egg Yolk Agar
|
M12
Anaerobic Egg Yolk Agar
| Yeast extract | 5 g |
| Tryptone | 5 g |
| Proteose peptone | 20 g |
| NaCl | 5 g |
| Agar | 20 g |
| Distilled water | 1 liter |
Autoclave 15 min at 121°C. Adjust pH to 7.0 ± 0.2.
Wash 2 fresh eggs with stiff brush and drain. Soak eggs in 70% ethanol for 1 h. Crack eggs aseptically. Retain yolks. Drain contents of yolk sacs into sterile stoppered graduate and discard sacs. Add yolk to equal volume of sterile 0.85% saline. Invert graduate several times to mix. Egg yolk emulsion (50%) is available commercially.
To 1 liter melted medium (48-50°C) add 80 ml yolk-saline mixture (available from Difco as Bacto Egg Yolk Enrichment 50%), and mix. Pour plates immediately. After solidification dry 2-3 days at ambient temperature or at 35°C for 24 h. Check plates for contamination before use. After drying, plates may be stored for a short period in refrigerator.
| Storage | 4°C |
Hypertext updated by rim/cjm 2005-OCT-21
