M116
OF Glucose Medium, Semisolid
|
M116
OF Glucose Medium, Semisolid
| Tryptone (trypticase) | 2 g |
| NaCl | 5 g |
| Dipotassium phosphate | 0.3 g |
| Bromthymol blue dye | 0.03 g |
| Agar | 2 g |
| Glucose | 10 g |
| Distilled water | 1 liter |
Boil ingredients to dissolve.
For halophilic Vibrio spp., add 15 g NaCl (2% NaCl, final concentration) to medium.
Dispense 5 ml into 13 x 100 mm tubes and autoclave 15 min at 121°C. Final pH, 6.8 ± 0.2.
*To use OF medium with sugars other than glucose, prepare medium without glucose in 900 ml water, sterilize as above, and cool to 45-50°C. Add 100 ml of 10% solution of filter-sterilized sugar to basal medium. Aseptically dispense 5 ml into sterile 13 x 100 mm tubes.
For Campylobacter, prepare half the tubes with glucose and half without.
For use with halophilic Vibrio spp., add NaCl to a final concentration of 2-3%.
Hypertext updated by rim/cjm 2005-OCT-21
