U.S. Food & Drug Administration
Center for Food Safety & Applied Nutrition

Bacteriological Analytical Manual Online
January 2001

M112
Nutrient Agar

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Beef extract 3 g
Peptone 5 g
Agar 15 g
Distilled water 1 liter

Heat to boiling to dissolve ingredients. Dispense into tubes or flasks. Autoclave 15 min at 121°C. Final pH, 6.8 ± 0.2. If used as base for blood agar, add 8 g NaCl to prevent hemolysis of blood cells.



Hypertext Source: Bacteriological Analytical Manual, 8th Edition, Revision A, 1998.

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