M11
Anaerobe Agar
|
M11
Anaerobe Agar
| Trypticase (tryptic) soy agar | 40 g |
| Agar | 5 g |
| Yeast extract | 5 g |
| L-Cysteine (dissolved in 5 ml 1 N NaOH) | 0.4 g |
| Distilled water | l liter |
Heat with agitation to dissolve agar. Adjust pH to 7.5 ± 0.2. Autoclave 15 min at 121°C. Cool to 50°C.
Suspend 1 g hemin in 100 ml distilled water. Autoclave 15 min at 121°C. Refrigerate at 4°C.
Dissolve 1 g vitamin K1 (Sigma Chemical Co., St. Louis, MO) in 100 ml 95% ethanol. Solution may require 2-3 days with intermittent shaking to dissolve. Refrigerate at 4°C.
To 1 liter base add 0.5 ml hemin solution and 1 ml Vitamin K1 solution. Mix and pour 20 ml portions into 15 x 100 mm petri dishes. Medium must be reduced before inoculation by 24 h anaerobic incubation in anaerobic glove box or GasPak jar.
Hypertext updated by rim/cjm 2005-OCT-21
