U.S. Food & Drug Administration
Center for Food Safety & Applied Nutrition

Bacteriological Analytical Manual Online
January 2001

M107
Mueller-Hinton Agar

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Beef, infusion from 300 g
Acidicase Peptone (BBL)
or
Casamino Acids (Difco)		 17.5 g
Starch 1.5 g
Agar 17 g
Distilled water 1 liter

 


Heat to boiling for 1 min. Autoclave 15 min at 116°C. Final pH, 7.3 ± 0.2.

 

 


For use with halophilic Vibrio spp., add NaCl to a final concentration of 2-3%.



Hypertext Source: Bacteriological Analytical Manual, 8th Edition, Revision A, 1998.

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Hypertext updated by rim/cjm 2005-OCT-21