U.S. Food & Drug Administration
Center for Food Safety & Applied Nutrition

Bacteriological Analytical Manual Online
January 2001

M105
Mucate Broth

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Peptone 10 g
Mucic acid 10 g
Bromthymol blue 0.024 g
Distilled water 1 liter

Dissolve peptone. Dissolve mucic acid by slowly adding 5 N NaOH and stirring. Dispense 5 ml portions into 13 x 100 mm screw-cap tubes. Autoclave 10 min at 121°C. Final pH, 7.4 ± 0.1.




Hypertext Source: Bacteriological Analytical Manual, 8th Edition, Revision A, 1998.


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