U.S. Food & Drug Administration
Center for Food Safety & Applied Nutrition

Bacteriological Analytical Manual Online
January 2001

M102
Motility-Nitrate Medium, Buffered
(for C. perfringens)

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Beef extract 3 g
Peptone (Difco) 5 g
KNO3 1 g
Na2HPO3 2.5 g
Agar 3 g
Galactose 5 g
Glycerin (reagent grade) 5 ml
Distilled water 1 liter

 

 


Dissolve all ingredients except agar. Adjust pH to 7.3 ± 0.1. Add agar and heat to dissolve. Dispense 11 ml portions into 16 x 150 mm tubes. Autoclave 15 min at 121°C. If not used within 4 h, heat 10 min in boiling water or flowing steam. Chill in cold water.



Hypertext Source: Bacteriological Analytical Manual, 8th Edition, Revision A, 1998.

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Hypertext updated by rim/cjm 2005-OCT-21