U.S. Food & Drug Administration
Center for Food Safety & Applied Nutrition

Bacteriological Analytical Manual Online
January 2001

M1
A-1 Medium


Tryptone  20 g
Lactose 5 g
NaCl 5 g
*Triton X-100 (Rohm & Haas) 1 ml
Salicin 0.5 g
Distilled water 1 liter

 

Dissolve ingredients in 1 liter distilled water. Adjust pH to 6.9 ± 0.1. Dispense 10 ml portions of single strength broth into 18 x 150 mm tubes containing inverted fermentation vials. For double strength broth, use 22 x 175 mm tubes containing inverted fermentation vials. Medium may be cloudy before sterilization. Autoclave 10 min at 121°C.


Store in dark up to 7 days.

(Commercially available A-1 medium is unacceptable.)

Storage dark, up to 7 days
*Triton X-100 may be purchased from Fisher Scientific Company, Fairlawn, NJ 07410.

Hypertext Source: Bacteriological Analytical Manual, 8th Edition, Revision A, 1998.

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Hypertext updated by rim/cjm 2005-OCT-21