U.S. Food & Drug Administration
Center for
Food
Safety &
Applied
Nutrition
Bacteriological
Analytical
Manual Online
January 2001
|
|
M114
Nutrient Broth
(Return to Table of Contents)
| Beef extract |
3 g |
| Peptone |
5 g |
| Distilled water |
1 liter |
Heat to dissolve. Dispense 10 m1 portions into tubes or 225 ml portions
into 500 ml Erlenmeyer flasks. Autoclave 15 min at 121°C. Final pH,
6.8 ± 0.2.
Hypertext Source: Bacteriological Analytical
Manual, 8th Edition, Revision A, 1998.
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Hypertext updated by rim/cjm 2005-OCT-21