|
| Trypticase peptone | 17 g |
| Phytone peptone | 3 g |
| NaCl | 5 g |
| K2HPO4 | 2.5 g |
| Glucose | 2.5 g |
| Distilled water | 1 liter |
Heat with gentle agitation to dissolve. Dispense 225 ml into 500 ml Erlenmeyer flasks. Autoclave 15 min at 121°C. Final pH, 7.3 ± 0.2.
For trypticase soy broth without glucose, prepare as above, but omit 2.5 g glucose.
For use with halophilic Vibrio spp., add NaCl to a final concentration of 2-3%.
Hypertext Source: Bacteriological Analytical Manual, 8th Edition, Revision A, 1998.
