M44
Decarboxylase Basal Medium
(Arginine, Lysine, Ornithine)
|
M44
Decarboxylase Basal Medium
(Arginine, Lysine, Ornithine)
| Peptone or gelysate | 5 g |
| Yeast extract | 3 g |
| Glucose | 1 g |
| Bromcresol purple | 0.02 g |
| Distilled water | 1 liter |
Adjust pH so that value after sterilization is 6.5 ± 0.2. Dispense 5 ml portions into 16 x 125 mm screw-cap tubes. Autoclave loosely capped tubes 10 min at 121°C. Screw the caps on tightly for storage and after inoculation. For control, use unsupplemented base.
| For | to 1 liter base, add: |
|---|---|
| Arginine Broth | 5 g L-arginine |
| Lysine Broth (Falkow) | 5 g L-lysine |
| Ornithine Broth | 5 g L-ornithine |
For use with halophilic Vibrio spp., add NaCl to a final concentration of 2-3%.
Hypertext updated by rim/ear/cjm 2005-OCT-21
