U.S. Food & Drug Administration
Center for Food Safety & Applied Nutrition

BacteriologicalAnalyticalManual Online
January 2001

M92
Malonate Broth

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Yeast extract 1 g
(NH4)2SO4 2 g
K2HPO4 0.6 g
KH2PO4 0.4 g
NaCl 2 g
Sodium malonate 3 g
Glucose 0.25 g
Bromthymol blue 0.025 g
Distilled water 1 liter

 

 

 

Dissolve by heating, if necessary. Dispense 3 ml portions into 13 x 100 mm test tubes. Autoclave 15 min at 121°C. Final pH, 6.7 ± 0.2.

 



Hypertext Source: Bacteriological Analytical Manual, 8th Edition, Revision A, 1998.

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