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Bacteriological Analytical Manual Online
May 2004

R90

Peptone-Tween-salt diluent (PTS)

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Peptone	1.0 g
Tween 80	10.0 g
Sodium chloride	30.0g
Distilled water	1 liter

Dissolve ingredients in distilled water, adjust pH to 7.2 and dispense into bottles of desired volume. Autoclave 15 min at 121°C.

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