Add 0.1 ml Spicer-Edwards EN Complex (Difco) to 0.07 ml horseradish peroxidase-protein A conjugate in 1 ml 1% gelatin-Tris-buffered saline (TBS), and stir at 4°C for 1 h. Dilute to 40 ml 1% gelatin-TBS. Use within a few hours. Sufficient for 4 hydrophobic grid membrane filters.

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