U.S. Food & Drug Administration
Center for Food Safety & Applied Nutrition

Bacteriological Analytical Manual Online
January 2001

R57
Peroxidase Substrate for Membrane ELISA

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Solution A


4-Chloro-1-naphthol 60 mg
Methanol (cold) 20 ml

Dissolve 4-chloro-1-naphthol in cold methanol. Store at -20°C.



Solution B


Hydrogen peroxide, 30% 60 1
Tris-buffered saline (TBS) 100 ml

Add 30% H2O2 to TBS at room temperature.



Immediately before use, mix ice cold Solution A with room temperature Solution B.



Hypertext Source: Bacteriological Analytical Manual, 8th Edition, Revision A, 1998.

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