Dispense 10 µl ascitic fluid into 1 ml 1% gelatin-Tris-buffered saline (TBS). Add 3 µl horseradish peroxidase-protein A conjugate, and stir mixture at 4°C for 1 h. Then dilute to 10 ml with 1% gelatin-TBS. Sufficient for 1 hydrophobic grid membrane filter.

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