RFE Fish Species Identification Isoelectric Focusing Tools

U.S. Food & Drug Administration
Seafood Products Research Center
Center for Food Safety & Applied Nutrition
Regulatory Fish Encyclopedia:
IEF Gel Lane Banding Pattern Peak Report Data

Peak Report Data from Isoelectric Focusing for a specimen of:
Yellowtail Snapper ---- Go to Display Gel Lane Navigator.

FDA Seafood List Scientific Name: Ocyurus chrysurus
FDA Seafood List Common Name: Yellowtail Snapper
FDA Seafood List Market Name: Snapper
Unique IEF Gel Code: 19940824gel02
Unique Web Code: ysh05Srr
Tissue Aliquot Code: ocyuchry H94-002 #01a Gel Analysis Conditions


Gel Image	Fish Image	Calibrated Gel

DISPLAY GEL LANE NAVIGATOR
RETURN TO TOP OF THIS DOCUMENT
Lane a pI Markers	Lane b Specimen	Lane c pI Markers	Lane d Co-Migrated	Lane e Specimen	Lane f pI Markers	Go to Gel Schematic

**DISPLAY GEL LANE LETTER:** Lane a
**DIGITAL REPORT GEL LANE:** 2
**LANE TYPE:** pI Markers
**ACTUAL GEL LANE:** 2
Go to Gel Schematic
Return to Lane Navigator
Band	pI	Loc	% RF	Amplitude	Width	Integr. OD	% Integr. OD	% Lane IntOD	pI	G. Loc	G. Sigma	G. Ampl
1	4.55	88	10.5	0.0971125	5	0.779471	10.8	8.4	4.55	88.3	4.8	0.0915996
2	5.2	184	22	0.108924	4	0.767775	10.6	8.3	5.2	183.6	4.1	0.105084
3	5.85	300	35.9	0.18179	3	0.865096	11.9	9.3	5.85	300.4	2.6	0.184878
4	6.55	431	51.6	0.0799246	8	1.09393	15.1	11.8	6.55	429.9	7.9	0.0777243
5	7.35	527	63.1	0.0410686	6	0.41652	5.7	4.5	7.35	526.6	6.2	0.0376383
6	8.15	641	76.8	0.0462623	8	0.65672	9.1	7.1	8.15	640.9	8.2	0.0450434
7	8.45	702	84.1	0.0801689	7	0.931914	12.9	10	8.45	701.2	6.7	0.0790358
8	8.65	751	89.9	0.0649285	4	0.440209	6.1	4.7	8.65	751	4	0.0618875
9	9.3	799	95.7	0.130623	6	1.2931	17.8	13.9	9.3	798.7	5.9	0.123671

PeakCounter: 9

**DISPLAY GEL LANE LETTER:** Lane b
**DIGITAL REPORT GEL LANE:** 3
**LANE TYPE:** Specimen
**ACTUAL GEL LANE:** 3
Go to Gel Schematic
Return to Lane Navigator
Band	pI	Loc	% RF	Amplitude	Width	Integr. OD	% Integr. OD	% Lane IntOD	pI	G. Loc	G. Sigma	G. Ampl
1	4.152	25	3	0.236471	6	2.42555	8.4	6.9	4.152	25.3	5.9	0.232576
2	4.805	126	15.1	0.195962	9	2.78555	9.6	7.9	4.805	128.8	9	0.175063
3	4.954	149	17.9	0.369821	7	4.17126	14.4	11.8	4.954	147.9	6.6	0.355511
4	5.401	220	26.4	0.011851	9	0.166422	0.6	0.5	5.401	216.2	8.7	0.0108481
5	5.505	237	28.4	0.0184471	3	0.110568	0.4	0.3	5.505	236.7	3.3	0.0186223
6	5.731	274	32.9	0.0483848	13	1.04501	3.6	3	5.731	272.3	12.8	0.0458954
7	5.925	308	36.9	0.0668085	5	0.486918	1.7	1.4	5.925	308.1	4.6	0.0599029
8	6.029	328	39.3	0.50722	3	2.70363	9.3	7.6	6.029	328.1	3.3	0.468021
9	6.091	340	40.8	0.398139	4	2.56804	8.9	7.3	6.091	340.5	3.8	0.378441
10	6.169	355	42.6	0.181211	5	1.55944	5.4	4.4	6.169	355	5.3	0.166413
Band	pI	Loc	% RF	Amplitude	Width	Integr. OD	% Integr. OD	% Lane IntOD	pI	G. Loc	G. Sigma	G. Ampl
11	6.2	361	43.3	0.169322	3	0.719016	2.5	2	6.2	362.2	2.9	0.138393
12	6.33	386	46.3	0.021895	3	0.117556	0.4	0.3	6.33	386.5	3.2	0.0206879
13	6.501	419	50.2	0.0976671	4	0.719971	2.5	2	6.501	419.2	4.3	0.0934007
14	6.596	434	52	0.0534542	2	0.21917	0.8	0.6	6.596	433	2.3	0.052822
15	6.67	443	53.1	0.433045	2	1.63531	5.6	4.6	6.67	443	2.2	0.419349
16	6.737	451	54.1	0.128022	4	0.848424	2.9	2.4	6.737	451.4	3.7	0.129627
17	6.836	463	55.5	0.132205	3	0.752167	2.6	2.1	6.836	463.3	3.3	0.129107
18	6.969	479	57.4	0.0957869	5	0.737347	2.5	2.1	6.969	478.4	4.7	0.0878262
19	7.093	494	59.2	0.269927	4	1.62754	5.6	4.6	7.093	494.2	3.6	0.258648
20	7.159	502	60.2	0.561992	4	3.56119	12.3	10.1	7.159	502.5	3.5	0.567617
Band	pI	Loc	% RF	Amplitude	Width	Integr. OD	% Integr. OD	% Lane IntOD	pI	G. Loc	G. Sigma	G. Ampl
21	7.267	515	61.8	0.0164612	2	0.0504664	0.2	0.1	7.267	515.2	1.8	0.0161289

PeakCounter: 21

**DISPLAY GEL LANE LETTER:** Lane c
**DIGITAL REPORT GEL LANE:** 4
**LANE TYPE:** pI Markers
**ACTUAL GEL LANE:** 4
Go to Gel Schematic
Return to Lane Navigator
Band	pI	Loc	% RF	Amplitude	Width	Integr. OD	% Integr. OD	% Lane IntOD	pI	G. Loc	G. Sigma	G. Ampl
1	4.55	86	10.3	0.0845051	5	0.680506	9.7	7.5	4.55	85.8	4.9	0.0785704
2	5.2	191	22.9	0.0984899	4	0.725641	10.3	8	5.2	191.5	4.4	0.0935966
3	5.85	288	34.5	0.135694	4	0.91147	13	10	5.85	288	3.8	0.133914
4	6.55	427	51.2	0.0717247	10	1.21427	17.3	13.3	6.55	424.7	9.6	0.0716919
5	7.35	524	62.8	0.042338	6	0.432551	6.2	4.7	7.35	523.4	6.2	0.0396604
6	8.15	635	76.1	0.054012	8	0.731815	10.4	8	8.15	635.8	8.1	0.0508649
7	8.45	697	83.6	0.067605	8	0.997102	14.2	10.9	8.45	697.5	8.4	0.0673011
8	8.65	746	89.4	0.0608986	4	0.398702	5.7	4.4	8.65	745.9	3.9	0.0572695
9	9.3	799	95.8	0.0813777	7	0.925392	13.2	10.1	9.3	798.7	6.6	0.0797028

PeakCounter: 9

**DISPLAY GEL LANE LETTER:** Lane d
**DIGITAL REPORT GEL LANE:** 5
**LANE TYPE:** Co-Migrated
**ACTUAL GEL LANE:** 5
Go to Gel Schematic
Return to Lane Navigator
Band	pI	Loc	% RF	Amplitude	Width	Integr. OD	% Integr. OD	% Lane IntOD	pI	G. Loc	G. Sigma	G. Ampl
1	4.151	32	3.8	0.155859	6	1.7542	6.6	5.8	4.151	32.4	6.4	0.153549
2	4.55	94	11.3	0.0412535	5	0.326218	1.2	1.1	4.55	94.5	4.7	0.0390001
3	4.795	132	15.8	0.184258	7	2.12853	8	7	4.795	133.6	7.1	0.168345
4	4.943	155	18.6	0.253997	8	3.41998	12.9	11.2	4.943	153.2	8.1	0.239025
5	5.2	195	23.4	0.0298511	4	0.191332	0.7	0.6	5.2	195.5	3.6	0.0297819
6	5.372	220	26.4	0.0130516	6	0.130543	0.5	0.4	5.372	218.7	5.9	0.0124001
7	5.454	232	27.9	0.0469157	3	0.239644	0.9	0.8	5.454	232.6	3	0.045472
8	5.708	269	32.3	0.0166258	8	0.206163	0.8	0.7	5.708	269.4	7.9	0.014689
9	5.903	300	36	0.0872954	6	0.843256	3.2	2.8	5.903	299.3	5.9	0.0810789
10	6.022	323	38.8	0.428088	4	3.14489	11.9	10.3	6.022	322.9	4.4	0.401705
Band	pI	Loc	% RF	Amplitude	Width	Integr. OD	% Integr. OD	% Lane IntOD	pI	G. Loc	G. Sigma	G. Ampl
11	6.104	339	40.7	0.248764	5	2.31764	8.8	7.6	6.104	338.2	5.5	0.23968
12	6.186	355	42.6	0.111478	6	1.10621	4.2	3.6	6.186	354.7	5.8	0.108518
13	6.222	362	43.5	0.0905591	4	0.508396	1.9	1.7	6.222	363	3.9	0.0743835
14	6.351	387	46.5	0.0066191	4	0.0511307	0.2	0.2	6.351	386.6	4.1	0.0070865
15	6.516	419	50.3	0.0781766	6	0.794605	3	2.6	6.516	419	6.3	0.0708149
16	6.684	442	53.1	0.286937	3	1.61376	6.1	5.3	6.684	441.7	3.3	0.275627
17	6.75	450	54	0.0794657	3	0.496939	1.9	1.6	6.75	450.1	3.4	0.0829254
18	6.832	460	55.2	0.0756985	4	0.517269	2	1.7	6.832	460.6	3.9	0.0743907
19	6.955	475	57	0.0497291	3	0.309323	1.2	1	6.955	474.8	3.4	0.0508826
20	7.145	498	59.8	0.488728	6	4.66741	17.6	15.3	7.145	496.5	5.8	0.454542
Band	pI	Loc	% RF	Amplitude	Width	Integr. OD	% Integr. OD	% Lane IntOD	pI	G. Loc	G. Sigma	G. Ampl
21	7.251	511	61.3	0.0153693	2	1.35E-5	0	0	7.251	511.7	1.6	4.61E-6
22	7.35	523	62.8	0.0190341	4	0.150877	0.6	0.5	7.35	523.5	4.2	0.0202874
23	8.15	637	76.5	0.0182049	7	0.228875	0.9	0.8	8.15	638.2	7.2	0.0179133
24	8.45	701	84.2	0.0215607	8	0.265666	1	0.9	8.45	702.3	7.8	0.019096
25	8.65	747	89.7	0.0458323	11	0.893822	3.4	2.9	8.65	751	11.4	0.0442488
26	9.3	796	95.6	0.0235194	4	0.177008	0.7	0.6	9.3	795.8	4.4	0.0226479

PeakCounter: 26

**DISPLAY GEL LANE LETTER:** Lane e
**DIGITAL REPORT GEL LANE:** 6
**LANE TYPE:** Specimen
**ACTUAL GEL LANE:** 6
Go to Gel Schematic
Return to Lane Navigator
Band	pI	Loc	% RF	Amplitude	Width	Integr. OD	% Integr. OD	% Lane IntOD	pI	G. Loc	G. Sigma	G. Ampl
1	4.169	32	3.9	0.233713	6	2.43989	8	6.4	4.169	33.3	6.1	0.226701
2	4.803	131	15.8	0.32159	6	3.04101	10	8	4.803	132.1	5.7	0.302336
3	4.957	155	18.7	0.255264	9	3.71407	12.2	9.8	4.957	153	8.8	0.238657
4	5.462	233	28	0.0663752	3	0.315436	1	0.8	5.462	233.1	2.7	0.0653276
5	5.691	268	32.3	0.0244859	5	0.199479	0.7	0.5	5.691	268.6	4.8	0.0234414
6	5.841	291	35	0.0219593	4	0.153874	0.5	0.4	5.841	290.1	4	0.0218866
7	5.89	300	36.1	0.074428	4	0.541739	1.8	1.4	5.89	300.8	4.4	0.0691566
8	6.027	326	39.2	0.613217	3	3.34454	11	8.8	6.027	326	3.2	0.587537
9	6.117	343	41.3	0.362623	4	2.47931	8.1	6.5	6.117	343.3	4.2	0.330028
10	6.201	359	43.2	0.152701	5	1.25166	4.1	3.3	6.201	359.8	4.9	0.145437
Band	pI	Loc	% RF	Amplitude	Width	Integr. OD	% Integr. OD	% Lane IntOD	pI	G. Loc	G. Sigma	G. Ampl
11	6.248	368	44.3	0.138645	3	0.662151	2.2	1.7	6.248	368	3	0.126608
12	6.369	391	47.1	0.0201965	3	0.10924	0.4	0.3	6.369	391.3	3.2	0.0191789
13	6.532	422	50.8	0.103667	5	0.880373	2.9	2.3	6.532	422.3	5.1	0.0980595
14	6.695	443	53.3	0.465362	2	1.83216	6	4.8	6.695	443	2.3	0.450152
15	6.768	452	54.4	0.141743	4	1.25838	4.1	3.3	6.768	450.2	4.3	0.163696
16	6.842	461	55.5	0.18264	5	1.58542	5.2	4.2	6.842	461.6	5.3	0.16807
17	6.949	474	57	0.140614	4	1.06041	3.5	2.8	6.949	473.8	4.5	0.134092
18	7.063	488	58.7	0.178513	5	2.32736	7.6	6.1	7.063	491.1	4.8	0.271406
19	7.145	498	59.9	0.589222	3	3.16384	10.4	8.4	7.145	498.1	3.2	0.564367
20	7.252	511	61.5	0.0229911	2	0.0962317	0.3	0.3	7.252	511.3	2.4	0.0224404

PeakCounter: 20

**DISPLAY GEL LANE LETTER:** Lane f
**DIGITAL REPORT GEL LANE:** 7
**LANE TYPE:** pI Markers
**ACTUAL GEL LANE:** 7
Go to Gel Schematic
Return to Lane Navigator
Band	pI	Loc	% RF	Amplitude	Width	Integr. OD	% Integr. OD	% Lane IntOD	pI	G. Loc	G. Sigma	G. Ampl
1	4.55	88	10.6	0.0845988	5	0.711708	8.8	6.9	4.55	88.6	5	0.0803864
2	5.2	190	22.8	0.131654	4	0.896798	11.1	8.7	5.2	189.8	4	0.125174
3	5.85	294	35.3	0.168719	3	1.01744	12.6	9.9	5.85	293.8	3.4	0.167479
4	6.55	424	51	0.0894445	7	1.11627	13.8	10.9	6.55	423.1	7.2	0.0868764
5	7.35	522	62.7	0.0549106	5	0.470105	5.8	4.6	7.35	521.8	5.3	0.0501961
6	8.15	634	76.2	0.0494813	10	0.879316	10.9	8.6	8.15	635.1	10.2	0.0486311
7	8.45	695	83.5	0.0890718	8	1.21498	15	11.8	8.45	695	7.8	0.0883448
8	8.65	750	90.1	0.0629352	4	0.443616	5.5	4.3	8.65	749.4	4.2	0.059882
9	9.3	803	96.5	0.141378	6	1.32316	16.4	12.9	9.3	802.8	5.5	0.134924

PeakCounter: 9

Gel Banding Patterns Display in HTML using GIF stacking technique
(Uses Migration Position Normalized Data from above text peak reports)
Return to Lane Navigator Go to Gel Schematic Key

a b c d e f

MK SP MK CO SP MK

pI 4.55 label for gel schematic

pI 5.20 label for gel schematic

pI 5.8 label for gel schematic

pI 6.55 label for gel schematic

pI 7.35 label for gel schematic

pI 8.15 label for gel schematic

pI 8.45 label for gel schematic

pI 8.65 label for gel schematic

pI 9.30 label for gel schematic

pI 4.55 label for gel schematic

pI 5.20 label for gel schematic

pI 5.8 label for gel schematic

pI 6.55 label for gel schematic

pI 7.35 label for gel schematic

pI 8.15 label for gel schematic

pI 8.45 label for gel schematic

pI 8.65 label for gel schematic

pI 9.30 label for gel schematic

Key to Gel Schematic:

MK=pI Marker Lanes, pI bands are in red
SP=Specimen Lanes, Specimen bands are in blue
CO=Co-migrated Lanes of pI Markers and Specimen (pI bands are in red, Specimen bands are in blue)
Note: In co-migrated lanes, some pI Markers and Specimen bands may overlap in experiment. Red pI
band is reported when overlapped peak position is equal to nominal pI Marker position. Blue Specimen
band is reported when overlapped peak position deviates slightly from nominal pI Marker position.
Weak (<2% rel. intensity), Medium (2-6% rel. intensity), and Strong (>6% rel. intensity) band intensities indicated by gel schematic band thickness.
Return to Top of Gel Schematic

PeakCount: 94

HTML Forms TextArea Normalized Peak-Position Peak-Area for Copy-Paste events
4.550 10.800 5.200 10.600 5.850 11.900 6.550 15.100 7.350 5.700 8.150 9.100 8.450 12.900 8.650 6.100 9.300 17.800 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0	DISPLAY GEL LANE LETTER: Lane a DIGITAL REPORT GEL LANE: 2 LANE TYPE: pI Markers ACTUAL GEL LANE: 2 PeakCount: 9 Return to Lane Navigator
4.152 8.400 4.805 9.600 4.954 14.400 5.401 0.600 5.505 0.400 5.731 3.600 5.925 1.700 6.029 9.300 6.091 8.900 6.169 5.400 6.200 2.500 6.330 0.400 6.501 2.500 6.596 0.800 6.670 5.600 6.737 2.900 6.836 2.600 6.969 2.500 7.093 5.600 7.159 12.300 7.267 0.200 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0	DISPLAY GEL LANE LETTER: Lane b DIGITAL REPORT GEL LANE: 3 LANE TYPE: Specimen ACTUAL GEL LANE: 3 PeakCount: 21 Return to Lane Navigator
4.550 9.700 5.200 10.300 5.850 13.000 6.550 17.300 7.350 6.200 8.150 10.400 8.450 14.200 8.650 5.700 9.300 13.200 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0	DISPLAY GEL LANE LETTER: Lane c DIGITAL REPORT GEL LANE: 4 LANE TYPE: pI Markers ACTUAL GEL LANE: 4 PeakCount: 9 Return to Lane Navigator
4.151 6.600 4.550 1.200 4.795 8.000 4.943 12.900 5.200 0.700 5.372 0.500 5.454 0.900 5.708 0.800 5.903 3.200 6.022 11.900 6.104 8.800 6.186 4.200 6.222 1.900 6.351 0.200 6.516 3.000 6.684 6.100 6.750 1.900 6.832 2.000 6.955 1.200 7.145 17.600 7.251 0 7.350 0.600 8.150 0.900 8.450 1.000 8.650 3.400 9.300 0.700 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0	DISPLAY GEL LANE LETTER: Lane d DIGITAL REPORT GEL LANE: 5 LANE TYPE: Co-Migrated ACTUAL GEL LANE: 5 PeakCount: 26 Return to Lane Navigator
4.169 8.000 4.803 10.000 4.957 12.200 5.462 1.000 5.691 0.700 5.841 0.500 5.890 1.800 6.027 11.000 6.117 8.100 6.201 4.100 6.248 2.200 6.369 0.400 6.532 2.900 6.695 6.000 6.768 4.100 6.842 5.200 6.949 3.500 7.063 7.600 7.145 10.400 7.252 0.300 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0	DISPLAY GEL LANE LETTER: Lane e DIGITAL REPORT GEL LANE: 6 LANE TYPE: Specimen ACTUAL GEL LANE: 6 PeakCount: 20 Return to Lane Navigator
4.550 8.800 5.200 11.100 5.850 12.600 6.550 13.800 7.350 5.800 8.150 10.900 8.450 15.000 8.650 5.500 9.300 16.400 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0	DISPLAY GEL LANE LETTER: Lane f DIGITAL REPORT GEL LANE: 7 LANE TYPE: pI Markers ACTUAL GEL LANE: 7 PeakCount: 9 Return to Lane Navigator

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EXPERIMENTAL CONDITIONS: Gels were scanned at 600 d.p.i. in Adobe Photoshop 3.02 using a Sharp JX-325 Scanner with Film Scanner Attachment. Gels were analyzed for Densitometry, Intregration, and Migration Position Normalization in EXCEL 5.0 Format. *.xls files were imported into Paradox 7.0 to save as *.db files.

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Delphi 4.0 Generation of this Isoelectric Focusing (IEF) RFE PAGE 5 Tools in HTML
This HTML is automatically generated from a series of Paradox 7.0 database filesof IEF peak reports is controlled with a main RFEIEF Paradox 7.0 database withDelphi 3.0 front end (see Delphi 2.0 form) and back end for facile addition and modification of the Content of these RFE PAGE 5 HTML tools.
Contact Brad Tenge at btenge@ora.fda.gov for details.

RFE Contacts
A summary list of RFE contact personnel is available at http://www.cfsan.fda.gov/~frf/rfecon.html.

For isoelectric focusing laboratory experiments for fish species identification, you may contact

Brad Tenge at E-mail: btenge@ora.fda.gov
Ngoc-Lan Nguyen at E-mail: nnguyen@ora.fda.gov
Jim Barnett at E-mail: jbarnett@ora.fda.gov
Pat Rogers at E-mail: pxr@cfsan.fda.gov

For isoelectric focusing gel analysis and pattern recognition applications, you may contact

Brad Tenge at E-mail: btenge@ora.fda.gov
Ngoc-Lan Nguyen at E-mail: nnguyen@ora.fda.gov
Fred Fry at E-mail frf@vm.cfsan.fda.gov

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Last updated on 2003-FEB-27 by bjt/frf/nln

Peak Report Data from Isoelectric Focusing for a specimen of: Yellowtail Snapper ---- Go to Display Gel Lane Navigator.

Peak Report Data from Isoelectric Focusing for a specimen of:
Yellowtail Snapper ---- Go to Display Gel Lane Navigator.