Fish Image
U.S. Food & Drug Administration
Seafood Products Research Center
Center for Food Safety & Applied Nutrition
Regulatory Fish Encyclopedia:
IEF Gel Lane Banding Pattern Peak Report Data
Gel Image

Peak Report Data from Isoelectric Focusing for a specimen of:
Nile Tilapia  ----  Go to Display Gel Lane Navigator.


FDA Seafood List Scientific Name: Oreochromis niloticus
FDA Seafood List Common Name: Nile Tilapia
FDA Seafood List Market Name: Tilapia
Unique IEF Gel Code: 19971028gel08
Unique Web Code: tnh01Srr
Tissue Aliquot Code: oreonilo I96-003 #02m Gel Analysis Conditions
Gel ImageFish ImageCalibrated Gel Image
Gel ImageFish ImageCalibrated Gel

DISPLAY GEL LANE NAVIGATOR
RETURN TO TOP OF THIS DOCUMENT
Lane a
pI Markers
Lane b
Specimen
Lane c
pI Markers
Lane d
Co-Migrated
Lane e
Specimen
Lane f
pI Markers
Go to Gel Schematic

 
DISPLAY GEL LANE LETTER: Lane a
DIGITAL REPORT GEL LANE: 2
LANE TYPE: pI Markers
ACTUAL GEL LANE: 2
Go to Gel Schematic
Return to Lane Navigator
BandpILoc% RFAmplitudeWidthIntegr. OD% Integr. OD% Lane IntODpIG. LocG. SigmaG. Ampl
14.558810.30.087286140.5407365.74.44.5588.23.70.0828211
25.218321.50.10173750.7928398.36.55.2183.94.90.0916108
35.8530035.20.16428430.86496197.15.85299.730.161582
46.5544251.90.13594471.723031814.16.55440.87.50.12963
56.8547355.50.05091360.496815.24.16.85474.75.70.0491237
67.3553963.30.14571441.0467910.98.57.35538.74.10.145368
78.1566077.50.0737573101.1640512.29.58.1566010.10.0648566
88.4572384.90.11047781.5081215.812.38.45722.77.80.109067
98.6577691.10.10592740.7213117.55.98.65775.840.102971
109.382997.30.08940750.7080077.45.89.3828.44.80.0836047
PeakCounter: 10
DISPLAY GEL LANE LETTER: Lane b
DIGITAL REPORT GEL LANE: 3
LANE TYPE: Specimen
ACTUAL GEL LANE: 3
Go to Gel Schematic
Return to Lane Navigator
BandpILoc% RFAmplitudeWidthIntegr. OD% Integr. OD% Lane IntODpIG. LocG. SigmaG. Ampl
14.092263.10.52140975.9350920.518.14.09226.56.60.506939
24.454758.80.40750543.2254311.29.84.45474.94.50.405741
35.36220524.10.059967940.3972741.41.25.362205.43.80.0594932
45.42921725.50.053076250.4395461.51.35.429217.25.20.0474532
55.5423727.90.53678532.69989.38.25.54237.630.505055
65.68526330.90.50644532.676119.38.25.685263.330.504416
75.74727432.20.20217551.562785.44.85.747273.84.60.190304
85.81928733.80.030680650.2354660.80.75.819289.84.50.0294313
95.88630035.30.11550930.4326041.51.35.886299.62.90.0835676
105.914306360.10946850.9796993.435.914305.85.20.106213
BandpILoc% RFAmplitudeWidthIntegr. OD% Integr. OD% Lane IntODpIG. LocG. SigmaG. Ampl
116.0934340.40.11261330.5141841.81.66.09343.32.50.115847
126.11934941.10.10843720.3956721.41.26.1193492.10.105977
136.15235641.90.084919930.3920371.41.26.152356.22.60.0839861
146.38140447.50.22441631.072723.73.36.3814042.70.224324
156.42441348.60.040587320.1338720.50.46.424412.81.80.0415549
166.44841849.20.080255620.3096491.10.96.448418.22.20.0787829
176.481425500.38713821.423464.94.36.481425.42.10.385294
186.63844952.80.4326642.90414108.96.638448.73.70.441583
196.73946054.10.024735110.05042770.20.26.739460.21.50.019096
206.84147155.40.010278520.0437830.20.16.8414712.40.0101401
BandpILoc% RFAmplitudeWidthIntegr. OD% Integr. OD% Lane IntODpIG. LocG. SigmaG. Ampl
217.38354163.60.031893950.28825310.97.383540.75.40.0298601
227.86461472.20.24104352.795059.78.57.8646175.10.309149
PeakCounter: 22
DISPLAY GEL LANE LETTER: Lane c
DIGITAL REPORT GEL LANE: 4
LANE TYPE: pI Markers
ACTUAL GEL LANE: 4
Go to Gel Schematic
Return to Lane Navigator
BandpILoc% RFAmplitudeWidthIntegr. OD% Integr. OD% Lane IntODpIG. LocG. SigmaG. Ampl
14.558910.50.058310540.3949964.63.44.5589.13.90.0568629
25.217020.10.052154870.5752536.755.2171.470.0464368
35.8528633.80.17110430.97548411.38.55.85286.33.20.172834
46.5543851.70.1472271.6608519.214.46.55437.26.80.137771
56.8547255.70.040053270.4424275.13.86.85472.96.60.0375874
67.35534630.12032340.8199189.57.17.35534.53.90.117438
78.1565677.40.078401881.0141111.78.88.15655.17.80.0736929
88.4571784.70.10035771.1917713.810.48.45716.66.80.0987271
98.6576990.80.093514440.6260037.35.48.657693.90.0913544
109.382497.30.13243640.92991110.88.19.3824.140.13029
PeakCounter: 10
DISPLAY GEL LANE LETTER: Lane d
DIGITAL REPORT GEL LANE: 5
LANE TYPE: Co-Migrated
ACTUAL GEL LANE: 5
Go to Gel Schematic
Return to Lane Navigator
BandpILoc% RFAmplitudeWidthIntegr. OD% Integr. OD% Lane IntODpIG. LocG. SigmaG. Ampl
14.038323.80.50843964.9987617.516.24.03832.75.80.490428
24.42829.60.4008742.757859.78.94.4282.53.90.399545
34.559911.60.063484630.3568921.31.24.5599.63.20.0638577
45.218421.60.050060870.5874052.11.95.2183.97.40.0449715
55.31720323.90.045138470.5038641.81.65.317202.86.60.0431431
65.37221224.90.037582640.2035630.70.75.372212.73.60.0317413
75.50123327.40.39048342.490968.78.15.501232.73.80.368331
85.692264310.427142.783579.895.692264.13.80.41557
95.77827832.70.17836571.906126.76.25.7782776.70.159966
105.89429935.10.19198893.0719310.89.95.894299.29.50.182809
BandpILoc% RFAmplitudeWidthIntegr. OD% Integr. OD% Lane IntODpIG. LocG. SigmaG. Ampl
116.06333539.40.064148330.3261951.11.16.06333530.061745
126.09234140.10.054801230.2410690.80.86.092340.62.60.0532071
136.1234740.80.048401820.1960540.70.66.12347.12.30.0479579
146.42141148.30.12311230.57208321.96.421411.32.60.125021
156.48342449.80.050509340.294401116.483424.73.50.0472799
166.5243250.80.29991321.19964.23.96.52431.92.30.295672
176.65945253.10.40196731.795986.35.86.659452.32.50.39767
186.7746654.80.012745230.06364340.20.26.77465.52.70.0130859
196.8547655.90.022090840.1484740.50.56.85476.53.80.0217819
207.3554163.60.072132140.4517261.61.57.35540.73.60.0706934
BandpILoc% RFAmplitudeWidthIntegr. OD% Integr. OD% Lane IntODpIG. LocG. SigmaG. Ampl
217.86561772.50.13582482.042577.26.67.865618.880.144098
228.1565977.40.020696150.2051360.70.78.15658.45.50.0210475
238.4572685.30.051484460.55974921.88.45725.160.0522727
248.65774910.048699130.296053118.65773.83.50.0479079
259.382697.10.065615840.432461.51.49.3826.13.90.0632804
PeakCounter: 25
DISPLAY GEL LANE LETTER: Lane e
DIGITAL REPORT GEL LANE: 6
LANE TYPE: Specimen
ACTUAL GEL LANE: 6
Go to Gel Schematic
Return to Lane Navigator
BandpILoc% RFAmplitudeWidthIntegr. OD% Integr. OD% Lane IntODpIG. LocG. SigmaG. Ampl
14.055283.30.4596464.8644319.517.24.055286.10.45348
24.4177790.27790262.8066511.39.94.41776.85.50.286656
35.30220023.50.041718370.4777991.91.75.302200.16.50.0411639
45.3520824.40.038357640.2222480.90.85.35209.140.0310894
55.4722826.80.29672652.861311.510.15.472285.40.299773
65.65625930.40.32160152.7666811.19.85.656258.74.90.318095
75.74627432.20.14015281.778657.16.35.746273.17.50.133145
85.89730135.40.155559102.425899.78.65.897300.59.60.142675
96.0633539.40.074817530.3433221.41.26.063352.70.0713716
106.08934140.10.062229520.2620421.10.96.089340.62.40.0613195
BandpILoc% RFAmplitudeWidthIntegr. OD% Integr. OD% Lane IntODpIG. LocG. SigmaG. Ampl
116.11834740.80.054659620.2335540.90.86.118347.52.30.0575168
126.41240847.90.16090830.8091063.22.96.412408.42.70.16673
136.455417490.022903310.05487990.20.26.455416.51.30.0235924
146.47942249.60.054295230.25027810.96.479421.92.70.0527184
156.50842850.30.34791721.452245.85.16.508428.22.30.35225
166.62544652.40.46645432.139018.67.66.6254462.70.450115
176.72945953.90.016924130.08192670.30.36.729458.42.70.0172263
186.81847055.20.0084955230.04653120.20.26.818470.23.40.00766003
197.34653863.20.019525830.11240.50.47.346538.43.40.0185097
207.85361472.20.11047950.9196773.73.27.853614.74.60.112148
PeakCounter: 20
DISPLAY GEL LANE LETTER: Lane f
DIGITAL REPORT GEL LANE: 7
LANE TYPE: pI Markers
ACTUAL GEL LANE: 7
Go to Gel Schematic
Return to Lane Navigator
BandpILoc% RFAmplitudeWidthIntegr. OD% Integr. OD% Lane IntODpIG. LocG. SigmaG. Ampl
14.558610.20.070005740.4396544.83.74.5586.43.60.0681834
25.217720.90.086772360.8462169.37.25.217760.0799001
35.85288340.15946741.00088118.55.85287.43.60.158138
46.5543050.80.13649192.0141222.217.16.55428.19.40.121407
56.8546755.20.042444570.5036245.54.36.85468.17.40.0385674
67.3553162.80.10613540.7183487.96.17.35531.240.101771
78.1565377.20.092327271.0757511.89.18.15651.66.80.0889631
88.45719850.072996981.0126411.18.68.45718.57.80.0735796
98.6576990.90.09068540.6587017.25.68.65768.54.30.0869116
109.382797.80.10006150.81958596.99.3825.94.80.0966977
PeakCounter: 10
Gel Banding Patterns Display in HTML using GIF stacking technique
(Uses Migration Position Normalized Data from above text peak reports)

Return to Lane Navigator    Go to Gel Schematic Key

a b c d e f
MK SP MK CO SP MK
white spacer for gel schematic
pI 4.55 label for gel schematic
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pI 5.20 label for gel schematic
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pI 5.8 label for gel schematic
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pI 6.55 label for gel schematic
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pI 8.15 label for gel schematic
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pI 8.45 label for gel schematic
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pI 4.55 label for gel schematic
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pI 5.20 label for gel schematic
white spacer for gel schematic
pI 5.8 label for gel schematic
white spacer for gel schematic
pI 6.55 label for gel schematic
white spacer for gel schematic
pI 6.85 label for gel schematic
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pI 7.35 label for gel schematic
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pI 8.15 label for gel schematic
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pI 8.45 label for gel schematic
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Key to Gel Schematic: MK=pI Marker Lanes, pI bands are in red
SP=Specimen Lanes, Specimen bands are in blue
CO=Co-migrated Lanes of pI Markers and Specimen (pI bands are in red, Specimen bands are in blue)
Note:  In co-migrated lanes, some pI Markers and Specimen bands may overlap in experiment.  Red pI
band is reported when overlapped peak position is equal to nominal pI Marker position.  Blue Specimen
band is reported when overlapped peak position deviates slightly from nominal pI Marker position.
Weak (<2% rel. intensity), Medium (2-6% rel. intensity), and Strong (>6% rel. intensity) band intensities indicated by gel schematic band thickness.
Return to Top of Gel Schematic

PeakCount: 97
HTML Forms TextArea Normalized Peak-Position Peak-Area for Copy-Paste events
DISPLAY GEL LANE LETTER: Lane a
DIGITAL REPORT GEL LANE: 2
LANE TYPE: pI Markers
ACTUAL GEL LANE: 2
PeakCount: 10 Return to Lane Navigator
DISPLAY GEL LANE LETTER: Lane b
DIGITAL REPORT GEL LANE: 3
LANE TYPE: Specimen
ACTUAL GEL LANE: 3
PeakCount: 22 Return to Lane Navigator
DISPLAY GEL LANE LETTER: Lane c
DIGITAL REPORT GEL LANE: 4
LANE TYPE: pI Markers
ACTUAL GEL LANE: 4
PeakCount: 10 Return to Lane Navigator
DISPLAY GEL LANE LETTER: Lane d
DIGITAL REPORT GEL LANE: 5
LANE TYPE: Co-Migrated
ACTUAL GEL LANE: 5
PeakCount: 25 Return to Lane Navigator
DISPLAY GEL LANE LETTER: Lane e
DIGITAL REPORT GEL LANE: 6
LANE TYPE: Specimen
ACTUAL GEL LANE: 6
PeakCount: 20 Return to Lane Navigator
DISPLAY GEL LANE LETTER: Lane f
DIGITAL REPORT GEL LANE: 7
LANE TYPE: pI Markers
ACTUAL GEL LANE: 7
PeakCount: 10 Return to Lane Navigator



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EXPERIMENTAL CONDITIONS: Gels were scanned at 600 d.p.i. in Adobe Photoshop 3.02 using a Sharp JX-325 Scanner with Film Scanner Attachment. Gels were analyzed for Densitometry, Intregration, and Migration Position Normalization in EXCEL 5.0 Format. *.xls files were imported into Paradox 7.0 to save as *.db files.

 RETURN TO TOP THIS DOCUMENT
Delphi 4.0 Generation of this Isoelectric Focusing (IEF) RFE PAGE 5 Tools in HTML
This HTML is automatically generated from a series of Paradox 7.0 database filesof IEF peak reports is controlled with a main RFEIEF Paradox 7.0 database withDelphi 3.0 front end (see Delphi 2.0 form) and back end for facile addition and modification of the Content of these RFE PAGE 5 HTML tools.
Contact Brad Tenge at btenge@ora.fda.gov for details.


RFE Contacts
A summary list of RFE contact personnel is available at http://www.cfsan.fda.gov/~frf/rfecon.html.

For isoelectric focusing laboratory experiments for fish species identification, you may contact

For isoelectric focusing gel analysis and pattern recognition applications, you may contact

For CFSAN web consultation and posting, you may contact


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Last updated on 2003-FEB-27 by bjt/frf/nln