RFE Fish Species Identification Isoelectric Focusing Tools

U.S. Food & Drug Administration
Seafood Products Research Center
Center for Food Safety & Applied Nutrition
Regulatory Fish Encyclopedia:
IEF Gel Lane Banding Pattern Peak Report Data

Peak Report Data from Isoelectric Focusing for a specimen of:
Red Snapper ---- Go to Display Gel Lane Navigator.

FDA Seafood List Scientific Name: Lutjanus campechanus
FDA Seafood List Common Name: Red Snapper
FDA Seafood List Market Name: Snapper
Unique IEF Gel Code: 19980130gel03
Unique Web Code: rsh04Srr
Tissue Aliquot Code: lutjcamp D97-004 #04m Gel Analysis Conditions


Gel Image	Fish Image	Calibrated Gel

DISPLAY GEL LANE NAVIGATOR
RETURN TO TOP OF THIS DOCUMENT
Lane a pI Markers	Lane b Specimen	Lane c pI Markers	Lane d Co-Migrated	Lane e Specimen	Lane f pI Markers	Go to Gel Schematic

**DISPLAY GEL LANE LETTER:** Lane a
**DIGITAL REPORT GEL LANE:** 2
**LANE TYPE:** pI Markers
**ACTUAL GEL LANE:** 2
Go to Gel Schematic
Return to Lane Navigator
Band	pI	Loc	% RF	Amplitude	Width	Integr. OD	% Integr. OD	% Lane IntOD	pI	G. Loc	G. Sigma	G. Ampl
1	4.55	68	8.6	0.0975527	5	0.781993	10.8	8.2	4.55	68.3	4.8	0.0915759
2	5.2	160	20.3	0.0937359	4	0.638373	8.9	6.7	5.2	160.2	4	0.0904126
3	5.85	269	34.2	0.120204	4	0.935982	13	9.8	5.85	269.3	4.3	0.122707
4	6.55	414	52.6	0.0750243	9	1.08581	15.1	11.4	6.55	412	8.6	0.0715714
5	6.85	450	57.2	0.0304523	6	0.280083	3.9	2.9	6.85	450.9	5.9	0.0266716
6	7.35	523	66.5	0.0737407	5	0.622711	8.6	6.6	7.35	522.3	5	0.0703511
7	8.15	644	81.8	0.0837195	8	1.07611	14.9	11.3	8.15	644.5	7.7	0.078832
8	8.45	709	90.1	0.105278	6	1.16165	16.1	12.2	8.45	708.1	6.4	0.102527
9	8.65	762	96.8	0.0814248	5	0.627416	8.7	6.6	8.65	761.7	4.6	0.0769977

PeakCounter: 9

**DISPLAY GEL LANE LETTER:** Lane b
**DIGITAL REPORT GEL LANE:** 3
**LANE TYPE:** Specimen
**ACTUAL GEL LANE:** 3
Go to Gel Schematic
Return to Lane Navigator
Band	pI	Loc	% RF	Amplitude	Width	Integr. OD	% Integr. OD	% Lane IntOD	pI	G. Loc	G. Sigma	G. Ampl
1	4.131	12	1.5	0.0538081	6	0.592069	2.1	1.8	4.131	11	6.3	0.0531791
2	4.348	42	5.3	0.186548	5	1.81569	6.6	5.4	4.348	41.9	5.4	0.188062
3	4.456	57	7.2	0.021498	8	0.246099	0.9	0.7	4.456	56.6	8.3	0.016691
4	4.723	94	11.9	0.0194985	5	0.183297	0.7	0.5	4.723	93.4	5.4	0.0191669
5	4.875	115	14.6	0.0227657	7	0.27191	1	0.8	4.875	114.1	6.5	0.023599
6	5.041	138	17.5	0.206594	5	1.48833	5.4	4.4	5.041	137.5	4.7	0.179751
7	5.206	161	20.5	0.0385494	18	1.13951	4.1	3.4	5.206	149.9	18	0.0357172
8	5.337	183	23.3	0.131074	4	0.855674	3.1	2.5	5.337	183.6	3.9	0.12453
9	5.687	242	30.7	0.0810283	8	1.1459	4.2	3.4	5.687	241.5	8.4	0.0767176
10	5.862	272	34.6	0.0121061	5	0.0974465	0.4	0.3	5.862	272	4.6	0.0118433
Band	pI	Loc	% RF	Amplitude	Width	Integr. OD	% Integr. OD	% Lane IntOD	pI	G. Loc	G. Sigma	G. Ampl
11	5.929	286	36.3	0.0173543	4	0.126637	0.5	0.4	5.929	286.3	4.2	0.0170511
12	6.04	309	39.3	0.387538	4	3.12953	11.4	9.3	6.04	309.3	4.5	0.39513
13	6.117	325	41.3	0.209567	5	2.0376	7.4	6.1	6.117	324.8	5.5	0.210301
14	6.218	346	44	0.174058	6	1.80196	6.5	5.4	6.218	345	5.9	0.171247
15	6.295	362	46	0.0333894	4	0.214934	0.8	0.6	6.295	363	3.8	0.0315325
16	6.377	379	48.2	0.0413935	4	0.305907	1.1	0.9	6.377	378.8	3.7	0.0462501
17	6.391	382	48.5	0.0359413	2	0.0881944	0.3	0.3	6.391	383.7	2.3	0.0214302
18	6.449	394	50.1	0.0193172	4	0.122976	0.4	0.4	6.449	393.8	3.6	0.0190564
19	6.483	401	51	0.0390099	4	0.276886	1	0.8	6.483	402	4	0.0391475
20	6.521	409	52	0.140573	3	0.834425	3	2.5	6.521	409.1	3.3	0.140892
Band	pI	Loc	% RF	Amplitude	Width	Integr. OD	% Integr. OD	% Lane IntOD	pI	G. Loc	G. Sigma	G. Ampl
21	6.675	430	54.6	0.446851	4	3.05035	11.1	9.1	6.675	429.7	3.9	0.445265
22	6.758	440	55.9	0.374148	4	2.39544	8.7	7.1	6.758	440.1	3.9	0.347655
23	6.885	456	57.9	0.149986	6	1.52865	5.6	4.5	6.885	455.2	6.3	0.136352
24	7.005	473	60.1	0.0128959	2	0.0382493	0.1	0.1	7.005	473	2.1	0.0102157
25	7.181	498	63.3	0.0121468	3	0.0596183	0.2	0.2	7.181	497.1	2.7	0.0125473
26	7.287	513	65.2	0.219959	4	1.36295	4.9	4	7.287	513.2	3.6	0.213013
27	7.691	574	72.9	0.0313323	7	0.359263	1.3	1.1	7.691	573	7.1	0.0285111
28	7.855	599	76.1	0.228874	5	1.97268	7.2	5.9	7.855	598.9	5.1	0.216734

PeakCounter: 28

**DISPLAY GEL LANE LETTER:** Lane c
**DIGITAL REPORT GEL LANE:** 4
**LANE TYPE:** pI Markers
**ACTUAL GEL LANE:** 4
Go to Gel Schematic
Return to Lane Navigator
Band	pI	Loc	% RF	Amplitude	Width	Integr. OD	% Integr. OD	% Lane IntOD	pI	G. Loc	G. Sigma	G. Ampl
1	4.55	71	9	0.0694248	6	0.778465	11.7	8.7	4.55	72.1	6.3	0.0693473
2	5.2	159	20.2	0.0426258	8	0.659475	9.9	7.4	5.2	155.7	8.3	0.0446074
3	5.85	269	34.2	0.0709456	7	0.888175	13.4	10	5.85	268.4	6.9	0.0724713
4	6.55	415	52.7	0.0912654	7	1.03907	15.6	11.7	6.55	413.6	7	0.0842047
5	6.85	451	57.3	0.0232184	6	0.215013	3.2	2.4	6.85	451.3	5.8	0.0208564
6	7.35	520	66.1	0.0594668	5	0.490992	7.4	5.5	7.35	520	4.7	0.0583261
7	8.15	643	81.7	0.0761755	7	0.924379	13.9	10.4	8.15	642.6	7.2	0.0728203
8	8.45	707	89.8	0.0876218	7	1.05781	15.9	11.9	8.45	705.9	7.1	0.0844441
9	8.65	760	96.6	0.0712881	5	0.586546	8.8	6.6	8.65	758.9	4.9	0.0681527

PeakCounter: 9

**DISPLAY GEL LANE LETTER:** Lane d
**DIGITAL REPORT GEL LANE:** 5
**LANE TYPE:** Co-Migrated
**ACTUAL GEL LANE:** 5
Go to Gel Schematic
Return to Lane Navigator
Band	pI	Loc	% RF	Amplitude	Width	Integr. OD	% Integr. OD	% Lane IntOD	pI	G. Loc	G. Sigma	G. Ampl
1	4.101	19	2.4	0.0340039	6	0.344179	1.1	1	4.101	18.2	5.7	0.0338991
2	4.326	48	6.1	0.149562	5	1.43763	4.6	4.2	4.326	48.2	5.4	0.1511
3	4.55	77	9.7	0.0584081	5	0.452696	1.5	1.3	4.55	77.8	4.5	0.0566614
4	4.713	98	12.4	0.0103339	5	0.0897299	0.3	0.3	4.713	97.6	4.8	0.0106501
5	4.844	115	14.6	0.0224048	6	0.22051	0.7	0.6	4.844	116.1	5.7	0.0219268
6	5.03	139	17.6	0.18556	6	1.75262	5.6	5.1	5.03	139.1	5.6	0.177039
7	5.2	161	20.4	0.0588591	10	0.874913	2.8	2.5	5.2	158	9.9	0.0497679
8	5.331	184	23.3	0.0949947	4	0.610433	2	1.8	5.331	183.8	3.9	0.0877679
9	5.645	239	30.3	0.0403062	12	0.844439	2.7	2.4	5.645	238	12.5	0.0381932
10	5.85	275	34.8	0.0690168	6	0.686695	2.2	2	5.85	274.6	5.8	0.0667203
Band	pI	Loc	% RF	Amplitude	Width	Integr. OD	% Integr. OD	% Lane IntOD	pI	G. Loc	G. Sigma	G. Ampl
11	6.035	312	39.5	0.316594	5	2.55347	8.2	7.4	6.035	311.4	4.6	0.314383
12	6.135	332	42	0.151572	5	1.38234	4.4	4	6.135	331.5	5.1	0.153447
13	6.23	351	44.4	0.171676	6	1.78795	5.8	5.2	6.23	350.7	5.8	0.173223
14	6.31	367	46.5	0.0397778	5	0.348021	1.1	1	6.31	366.7	4.7	0.041503
15	6.41	387	49	0.0525987	6	0.531945	1.7	1.5	6.41	386.1	5.6	0.0534791
16	6.47	399	50.5	0.0591049	4	0.418134	1.3	1.2	6.47	398.5	4.2	0.0560962
17	6.51	407	51.5	0.0595327	3	0.305478	1	0.9	6.51	405.9	3.2	0.0539659
18	6.54	413	52.3	0.157353	3	0.990182	3.2	2.9	6.54	412.7	3.5	0.159875
19	6.682	431	54.6	0.456059	4	2.92987	9.4	8.5	6.682	431.2	3.6	0.457698
20	6.765	441	55.8	0.388782	4	2.34147	7.5	6.8	6.765	441.2	3.5	0.375666
Band	pI	Loc	% RF	Amplitude	Width	Integr. OD	% Integr. OD	% Lane IntOD	pI	G. Loc	G. Sigma	G. Ampl
21	6.875	455	57.6	0.146102	5	1.33819	4.3	3.9	6.875	455	5.4	0.139289
22	6.999	473	59.9	0.0061698	2	0.0276619	0.1	0.1	6.999	473.3	2.4	0.0064255
23	7.157	496	62.8	0.0092231	3	0.0421804	0.1	0.1	7.157	496.9	2.8	0.0085444
24	7.267	512	64.8	0.190385	4	1.18377	3.8	3.4	7.267	512.2	3.6	0.1861
25	7.35	524	66.3	0.046808	5	0.512397	1.6	1.5	7.35	521.5	4.9	0.058929
26	7.649	570	72.2	0.0693119	13	1.97079	6.3	5.7	7.649	575.9	13.4	0.082743
27	7.831	598	75.7	0.297675	9	4.12285	13.3	11.9	7.831	599.1	9	0.258427
28	8.15	647	81.9	0.0161754	5	0.149849	0.5	0.4	8.15	648.9	5.1	0.016437
29	8.45	711	90	0.0536591	5	0.493925	1.6	1.4	8.45	711.5	5.1	0.0549624
30	8.65	762	96.5	0.0427141	5	0.33092	1.1	1	8.65	761.9	4.5	0.0410461

PeakCounter: 30

**DISPLAY GEL LANE LETTER:** Lane e
**DIGITAL REPORT GEL LANE:** 6
**LANE TYPE:** Specimen
**ACTUAL GEL LANE:** 6
Go to Gel Schematic
Return to Lane Navigator
Band	pI	Loc	% RF	Amplitude	Width	Integr. OD	% Integr. OD	% Lane IntOD	pI	G. Loc	G. Sigma	G. Ampl
1	4.078	9	1.1	0.057663	7	0.668555	2.4	2.2	4.078	8.9	6.8	0.0552325
2	4.294	38	4.8	0.174252	6	1.77732	6.4	5.7	4.294	38.2	5.8	0.171505
3	4.39	51	6.5	0.0182509	9	0.31566	1.1	1	4.39	48.7	9.4	0.018911
4	4.68	90	11.4	0.013592	6	0.135373	0.5	0.4	4.68	89.3	5.7	0.0133339
5	4.821	109	13.9	0.0209974	5	0.173789	0.6	0.6	4.821	108.6	4.8	0.0204981
6	4.985	131	16.6	0.142879	6	1.49109	5.4	4.8	4.985	131.2	6	0.139152
7	5.133	151	19.2	0.0286325	12	0.646844	2.3	2.1	5.133	147	12.5	0.0293111
8	5.283	174	22.1	0.07351	6	0.818629	3	2.6	5.283	173.6	6.2	0.074947
9	5.62	231	29.4	0.0812397	10	1.34124	4.9	4.3	5.62	229.1	9.8	0.0772477
10	5.791	260	33	0.0028952	2	0.0077332	0	0	5.791	260.3	1.6	0.002676
Band	pI	Loc	% RF	Amplitude	Width	Integr. OD	% Integr. OD	% Lane IntOD	pI	G. Loc	G. Sigma	G. Ampl
11	5.88	276	35.1	0.0119601	6	0.129801	0.5	0.4	5.88	274.3	6.2	0.0117302
12	6	300	38.1	0.277481	6	3.18146	11.5	10.3	6	298.6	6.1	0.292824
13	6.085	317	40.3	0.167063	6	1.94103	7	6.3	6.085	316.8	6.3	0.174196
14	6.18	336	42.7	0.167701	7	1.99567	7.2	6.4	6.18	335.3	6.7	0.167123
15	6.26	352	44.7	0.0153121	3	0.0647108	0.2	0.2	6.26	354.5	3.1	0.0118936
16	6.365	373	47.4	0.0451774	5	0.367003	1.3	1.2	6.365	373	4.8	0.0427913
17	6.395	379	48.2	0.0190109	2	0.0342532	0.1	0.1	6.395	378.6	1.8	0.010873
18	6.44	388	49.3	0.0089113	4	0.0669341	0.2	0.2	6.44	389.7	4.5	0.0084681
19	6.48	396	50.3	0.0144939	3	0.13601	0.5	0.4	6.48	398.9	3	0.0252891
20	6.525	405	51.5	0.125057	3	0.728963	2.6	2.4	6.525	404.9	3.3	0.126053
Band	pI	Loc	% RF	Amplitude	Width	Integr. OD	% Integr. OD	% Lane IntOD	pI	G. Loc	G. Sigma	G. Ampl
21	6.673	425	54	0.373195	4	2.7073	9.8	8.7	6.673	424.9	4.2	0.366395
22	6.755	435	55.3	0.333424	4	2.05487	7.4	6.6	6.755	434.9	3.6	0.318372
23	6.873	450	57.2	0.14174	5	1.2637	4.6	4.1	6.873	449.3	5.4	0.13255
24	6.971	464	59	0.0061467	2	0.0196289	0.1	0.1	6.971	465.9	2.3	0.0048663
25	7.154	490	62.3	0.0085252	3	0.0472382	0.2	0.2	7.154	489.1	3.3	0.0081095
26	7.266	506	64.3	0.223657	4	1.65639	6	5.4	7.266	505.7	4.3	0.217351
27	7.675	569	72.3	0.0224486	3	0.13657	0.5	0.4	7.675	568.1	3.5	0.0221554
28	7.828	593	75.3	0.282337	8	3.6993	13.4	12	7.828	591.9	7.7	0.270979

PeakCounter: 28

**DISPLAY GEL LANE LETTER:** Lane f
**DIGITAL REPORT GEL LANE:** 7
**LANE TYPE:** pI Markers
**ACTUAL GEL LANE:** 7
Go to Gel Schematic
Return to Lane Navigator
Band	pI	Loc	% RF	Amplitude	Width	Integr. OD	% Integr. OD	% Lane IntOD	pI	G. Loc	G. Sigma	G. Ampl
1	4.55	72	9.1	0.105014	6	1.1453	12.3	9.6	4.55	72.8	6.5	0.0996084
2	5.2	163	20.7	0.0734546	7	0.833167	9	7	5.2	162.1	6.6	0.0715607
3	5.85	269	34.1	0.103998	5	0.983197	10.6	8.2	5.85	268.3	5.3	0.103924
4	6.55	409	51.8	0.109009	7	1.24646	13.4	10.4	6.55	407.9	6.7	0.104277
5	6.85	446	56.5	0.0389513	7	0.426927	4.6	3.6	6.85	446.4	6.9	0.0350084
6	7.35	516	65.4	0.106084	5	0.867758	9.3	7.3	7.35	515.9	4.8	0.101982
7	8.15	644	81.6	0.0765558	11	1.33262	14.3	11.1	8.15	643.5	10.6	0.0708386
8	8.45	705	89.4	0.132162	7	1.59359	17.1	13.3	8.45	704.6	7.1	0.12624
9	8.65	758	96.1	0.101521	5	0.86454	9.3	7.2	8.65	757.5	4.9	0.0992403

PeakCounter: 9

Gel Banding Patterns Display in HTML using GIF stacking technique
(Uses Migration Position Normalized Data from above text peak reports)
Return to Lane Navigator Go to Gel Schematic Key

a b c d e f

MK SP MK CO SP MK

pI 4.55 label for gel schematic

pI 5.20 label for gel schematic

pI 5.8 label for gel schematic

pI 6.55 label for gel schematic

pI 6.85 label for gel schematic

pI 7.35 label for gel schematic

pI 8.15 label for gel schematic

pI 8.45 label for gel schematic

pI 8.65 label for gel schematic

pI 4.55 label for gel schematic

pI 5.20 label for gel schematic

pI 5.8 label for gel schematic

pI 6.55 label for gel schematic

pI 6.85 label for gel schematic

pI 7.35 label for gel schematic

pI 8.15 label for gel schematic

pI 8.45 label for gel schematic

pI 8.65 label for gel schematic

Key to Gel Schematic:

MK=pI Marker Lanes, pI bands are in red
SP=Specimen Lanes, Specimen bands are in blue
CO=Co-migrated Lanes of pI Markers and Specimen (pI bands are in red, Specimen bands are in blue)
Note: In co-migrated lanes, some pI Markers and Specimen bands may overlap in experiment. Red pI
band is reported when overlapped peak position is equal to nominal pI Marker position. Blue Specimen
band is reported when overlapped peak position deviates slightly from nominal pI Marker position.
Weak (<2% rel. intensity), Medium (2-6% rel. intensity), and Strong (>6% rel. intensity) band intensities indicated by gel schematic band thickness.
Return to Top of Gel Schematic

PeakCount: 113

HTML Forms TextArea Normalized Peak-Position Peak-Area for Copy-Paste events
4.550 10.800 5.200 8.900 5.850 13.000 6.550 15.100 6.850 3.900 7.350 8.600 8.150 14.900 8.450 16.100 8.650 8.700 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0	DISPLAY GEL LANE LETTER: Lane a DIGITAL REPORT GEL LANE: 2 LANE TYPE: pI Markers ACTUAL GEL LANE: 2 PeakCount: 9 Return to Lane Navigator
4.131 2.100 4.348 6.600 4.456 0.900 4.723 0.700 4.875 1.000 5.041 5.400 5.206 4.100 5.337 3.100 5.687 4.200 5.862 0.400 5.929 0.500 6.040 11.400 6.117 7.400 6.218 6.500 6.295 0.800 6.377 1.100 6.391 0.300 6.449 0.400 6.483 1.000 6.521 3.000 6.675 11.100 6.758 8.700 6.885 5.600 7.005 0.100 7.181 0.200 7.287 4.900 7.691 1.300 7.855 7.200 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0	DISPLAY GEL LANE LETTER: Lane b DIGITAL REPORT GEL LANE: 3 LANE TYPE: Specimen ACTUAL GEL LANE: 3 PeakCount: 28 Return to Lane Navigator
4.550 11.700 5.200 9.900 5.850 13.400 6.550 15.600 6.850 3.200 7.350 7.400 8.150 13.900 8.450 15.900 8.650 8.800 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0	DISPLAY GEL LANE LETTER: Lane c DIGITAL REPORT GEL LANE: 4 LANE TYPE: pI Markers ACTUAL GEL LANE: 4 PeakCount: 9 Return to Lane Navigator
4.101 1.100 4.326 4.600 4.550 1.500 4.713 0.300 4.844 0.700 5.030 5.600 5.200 2.800 5.331 2.000 5.645 2.700 5.850 2.200 6.035 8.200 6.135 4.400 6.230 5.800 6.310 1.100 6.410 1.700 6.470 1.300 6.510 1.000 6.540 3.200 6.682 9.400 6.765 7.500 6.875 4.300 6.999 0.100 7.157 0.100 7.267 3.800 7.350 1.600 7.649 6.300 7.831 13.300 8.150 0.500 8.450 1.600 8.650 1.100 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0	DISPLAY GEL LANE LETTER: Lane d DIGITAL REPORT GEL LANE: 5 LANE TYPE: Co-Migrated ACTUAL GEL LANE: 5 PeakCount: 30 Return to Lane Navigator
4.078 2.400 4.294 6.400 4.390 1.100 4.680 0.500 4.821 0.600 4.985 5.400 5.133 2.300 5.283 3.000 5.620 4.900 5.791 0 5.880 0.500 6.000 11.500 6.085 7.000 6.180 7.200 6.260 0.200 6.365 1.300 6.395 0.100 6.440 0.200 6.480 0.500 6.525 2.600 6.673 9.800 6.755 7.400 6.873 4.600 6.971 0.100 7.154 0.200 7.266 6.000 7.675 0.500 7.828 13.400 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0	DISPLAY GEL LANE LETTER: Lane e DIGITAL REPORT GEL LANE: 6 LANE TYPE: Specimen ACTUAL GEL LANE: 6 PeakCount: 28 Return to Lane Navigator
4.550 12.300 5.200 9.000 5.850 10.600 6.550 13.400 6.850 4.600 7.350 9.300 8.150 14.300 8.450 17.100 8.650 9.300 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0	DISPLAY GEL LANE LETTER: Lane f DIGITAL REPORT GEL LANE: 7 LANE TYPE: pI Markers ACTUAL GEL LANE: 7 PeakCount: 9 Return to Lane Navigator

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EXPERIMENTAL CONDITIONS: Gels were scanned at 600 d.p.i. in Adobe Photoshop 3.02 using a Sharp JX-325 Scanner with Film Scanner Attachment. Gels were analyzed for Densitometry, Intregration, and Migration Position Normalization in EXCEL 5.0 Format. *.xls files were imported into Paradox 7.0 to save as *.db files.

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Delphi 4.0 Generation of this Isoelectric Focusing (IEF) RFE PAGE 5 Tools in HTML
This HTML is automatically generated from a series of Paradox 7.0 database filesof IEF peak reports is controlled with a main RFEIEF Paradox 7.0 database withDelphi 3.0 front end (see Delphi 2.0 form) and back end for facile addition and modification of the Content of these RFE PAGE 5 HTML tools.
Contact Brad Tenge at btenge@ora.fda.gov for details.

RFE Contacts
A summary list of RFE contact personnel is available at http://www.cfsan.fda.gov/~frf/rfecon.html.

For isoelectric focusing laboratory experiments for fish species identification, you may contact

Brad Tenge at E-mail: btenge@ora.fda.gov
Ngoc-Lan Nguyen at E-mail: nnguyen@ora.fda.gov
Jim Barnett at E-mail: jbarnett@ora.fda.gov
Pat Rogers at E-mail: pxr@cfsan.fda.gov

For isoelectric focusing gel analysis and pattern recognition applications, you may contact

Brad Tenge at E-mail: btenge@ora.fda.gov
Ngoc-Lan Nguyen at E-mail: nnguyen@ora.fda.gov
Fred Fry at E-mail frf@vm.cfsan.fda.gov

For CFSAN web consultation and posting, you may contact

Fred Fry E-mail: frf@vm.cfsan.fda.gov

Thank you for using the Regulatory Fish Encyclopedia (RFE)!

RFE Species Browse | RFE Tutorial | Seafoods | CFSAN

Last updated on 2003-FEB-27 by bjt/frf/nln

Peak Report Data from Isoelectric Focusing for a specimen of: Red Snapper ---- Go to Display Gel Lane Navigator.

Peak Report Data from Isoelectric Focusing for a specimen of:
Red Snapper ---- Go to Display Gel Lane Navigator.