![]() | Seafood Products Research Center Center for Food Safety & Applied Nutrition Regulatory Fish Encyclopedia: IEF Gel Lane Banding Pattern Peak Report Data | ![]() |
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Gel Image | Fish Image | Calibrated Gel | Comparison Gel |
Lane a pI Markers |
Lane b Co-Migrated |
Lane c Specimen |
Lane d pI Markers |
Lane e Co-Migrated |
Lane f Specimen |
Lane g pI Markers |
Go to Gel Schematic |
Band | pI | Loc | % RF | Amplitude | Width | Integr. OD | % Integr. OD | % Lane IntOD | pI | G. Loc | G. Sigma | G. Ampl |
1 | 3.5 | 60 | 5.1 | 0.260332 | 19 | 8.04519 | 30.8 | 26.3 | 3.5 | 53.2 | 19.2 | 0.236126 |
2 | 4.55 | 207 | 17.5 | 0.360163 | 3 | 1.86285 | 7.1 | 6.1 | 4.55 | 206.7 | 3.1 | 0.336917 |
3 | 5.2 | 287 | 24.3 | 0.410366 | 4 | 2.54981 | 9.8 | 8.3 | 5.2 | 286.8 | 3.6 | 0.396662 |
4 | 5.85 | 428 | 36.2 | 0.41107 | 3 | 1.90092 | 7.3 | 6.2 | 5.85 | 428.2 | 2.6 | 0.410684 |
5 | 6.55 | 601 | 50.9 | 0.303896 | 4 | 2.31145 | 8.8 | 7.6 | 6.55 | 600.2 | 4.4 | 0.293134 |
6 | 6.85 | 638 | 54 | 0.0655465 | 6 | 0.605878 | 2.3 | 2 | 6.85 | 638.6 | 5.8 | 0.0589054 |
7 | 7.35 | 734 | 62.2 | 0.229867 | 5 | 1.88408 | 7.2 | 6.2 | 7.35 | 733.3 | 4.9 | 0.21582 |
8 | 8.15 | 894 | 75.7 | 0.0546466 | 9 | 0.84412 | 3.2 | 2.8 | 8.15 | 893.7 | 9.1 | 0.0523628 |
9 | 8.45 | 960 | 81.3 | 0.0702827 | 6 | 0.752282 | 2.9 | 2.5 | 8.45 | 960.2 | 6.1 | 0.0692491 |
10 | 8.65 | 1001 | 84.8 | 0.0591252 | 5 | 0.529738 | 2 | 1.7 | 8.65 | 1000.5 | 5.1 | 0.0583328 |
Band | pI | Loc | % RF | Amplitude | Width | Integr. OD | % Integr. OD | % Lane IntOD | pI | G. Loc | G. Sigma | G. Ampl |
11 | 9.3 | 1127 | 95.4 | 0.336464 | 9 | 4.85316 | 18.6 | 15.9 | 9.3 | 1124.7 | 8.8 | 0.312087 |
Band | pI | Loc | % RF | Amplitude | Width | Integr. OD | % Integr. OD | % Lane IntOD | pI | G. Loc | G. Sigma | G. Ampl |
1 | 3.5 | 62 | 5.3 | 0.236777 | 15 | 5.82441 | 11.6 | 9.3 | 3.5 | 59.3 | 15 | 0.219685 |
2 | 3.704 | 91 | 7.7 | 0.103298 | 9 | 1.64825 | 3.3 | 2.6 | 3.704 | 89.9 | 8.9 | 0.104221 |
3 | 4.303 | 176 | 14.9 | 0.509048 | 4 | 3.50856 | 7 | 5.6 | 4.303 | 175.7 | 3.9 | 0.513614 |
4 | 4.487 | 202 | 17.1 | 0.0763168 | 7 | 0.903324 | 1.8 | 1.4 | 4.487 | 201.8 | 7.3 | 0.0696085 |
5 | 4.55 | 211 | 17.9 | 0.227864 | 5 | 1.57582 | 3.1 | 2.5 | 4.55 | 210.3 | 4.6 | 0.194637 |
6 | 4.658 | 224 | 19 | 0.0145884 | 4 | 0.198078 | 0.4 | 0.3 | 4.658 | 220.5 | 4.2 | 0.0268652 |
7 | 4.825 | 244 | 20.7 | 0.130308 | 6 | 1.10677 | 2.2 | 1.8 | 4.825 | 243.1 | 5.6 | 0.111344 |
8 | 4.942 | 258 | 21.9 | 0.00710838 | 2 | 0.0205415 | 0 | 0 | 4.942 | 259.3 | 2 | 0.00592637 |
9 | 5.092 | 276 | 23.4 | 0.110486 | 7 | 1.34414 | 2.7 | 2.2 | 5.092 | 274.8 | 7.4 | 0.101803 |
10 | 5.2 | 289 | 24.5 | 0.221064 | 6 | 2.18988 | 4.4 | 3.5 | 5.2 | 288.5 | 6.2 | 0.200333 |
Band | pI | Loc | % RF | Amplitude | Width | Integr. OD | % Integr. OD | % Lane IntOD | pI | G. Loc | G. Sigma | G. Ampl |
11 | 5.268 | 303 | 25.7 | 0.514541 | 4 | 3.20699 | 6.4 | 5.1 | 5.268 | 302.8 | 3.7 | 0.485033 |
12 | 5.321 | 314 | 26.6 | 0.31591 | 5 | 2.42694 | 4.8 | 3.9 | 5.321 | 314.2 | 4.8 | 0.283262 |
13 | 5.423 | 335 | 28.4 | 0.114221 | 3 | 0.642122 | 1.3 | 1 | 5.423 | 335.1 | 3.2 | 0.112146 |
14 | 5.535 | 358 | 30.3 | 0.0153233 | 2 | 0.0459598 | 0.1 | 0.1 | 5.535 | 357.8 | 1.7 | 0.0151857 |
15 | 5.583 | 368 | 31.2 | 0.00416545 | 1 | 0 | 0 | 0 | 5.583 | 362 | 1 | 0 |
16 | 5.632 | 378 | 32 | 0.574516 | 3 | 3.20422 | 6.4 | 5.1 | 5.632 | 378.7 | 3.3 | 0.545325 |
17 | 5.724 | 397 | 33.6 | 0.287733 | 5 | 2.27722 | 4.5 | 3.7 | 5.724 | 397.7 | 4.6 | 0.279837 |
18 | 5.85 | 423 | 35.8 | 0.120757 | 4 | 0.892867 | 1.8 | 1.4 | 5.85 | 422.9 | 4.3 | 0.116492 |
19 | 5.954 | 449 | 38.1 | 0.066875 | 11 | 1.12428 | 2.2 | 1.8 | 5.954 | 450.5 | 11 | 0.0574198 |
20 | 6.062 | 476 | 40.3 | 0.249413 | 2 | 1.04739 | 2.1 | 1.7 | 6.062 | 475.6 | 2.4 | 0.247881 |
Band | pI | Loc | % RF | Amplitude | Width | Integr. OD | % Integr. OD | % Lane IntOD | pI | G. Loc | G. Sigma | G. Ampl |
21 | 6.102 | 486 | 41.2 | 0.00307961 | 1 | 0 | 0 | 0 | 6.102 | 466 | 1 | 0 |
22 | 6.142 | 496 | 42 | 0.149065 | 2 | 0.577125 | 1.1 | 0.9 | 6.142 | 496.1 | 2.2 | 0.151419 |
23 | 6.174 | 504 | 42.7 | 0.244246 | 2 | 0.930794 | 1.9 | 1.5 | 6.174 | 504.1 | 2.1 | 0.246791 |
24 | 6.222 | 516 | 43.7 | 0.0244139 | 6 | 0.234332 | 0.5 | 0.4 | 6.222 | 515.8 | 5.7 | 0.0233325 |
25 | 6.306 | 537 | 45.5 | 0.0728528 | 10 | 1.26622 | 2.5 | 2 | 6.306 | 536.9 | 10.1 | 0.0707063 |
26 | 6.414 | 564 | 47.8 | 0.0155913 | 7 | 0.136817 | 0.3 | 0.2 | 6.414 | 566.1 | 6.6 | 0.0116134 |
27 | 6.55 | 598 | 50.7 | 0.18115 | 5 | 1.34687 | 2.7 | 2.2 | 6.55 | 597.2 | 4.6 | 0.166749 |
28 | 6.85 | 636 | 53.9 | 0.0435047 | 3 | 0.235156 | 0.5 | 0.4 | 6.85 | 636.1 | 3.3 | 0.040336 |
29 | 7.35 | 731 | 61.9 | 0.282097 | 6 | 2.72782 | 5.4 | 4.4 | 7.35 | 729.7 | 5.9 | 0.261533 |
30 | 7.456 | 752 | 63.7 | 0.0434563 | 10 | 0.727868 | 1.4 | 1.2 | 7.456 | 751.3 | 9.9 | 0.0415078 |
Band | pI | Loc | % RF | Amplitude | Width | Integr. OD | % Integr. OD | % Lane IntOD | pI | G. Loc | G. Sigma | G. Ampl |
31 | 8.15 | 889 | 75.3 | 0.619091 | 2 | 2.19833 | 4.4 | 3.5 | 8.15 | 889.7 | 2 | 0.618728 |
32 | 8.45 | 954 | 80.8 | 0.0745229 | 7 | 0.930888 | 1.9 | 1.5 | 8.45 | 954.1 | 7.3 | 0.0719806 |
33 | 8.65 | 998 | 84.6 | 0.0749047 | 5 | 0.604632 | 1.2 | 1 | 8.65 | 997.3 | 4.8 | 0.0717231 |
34 | 9.3 | 1134 | 96.1 | 0.393312 | 9 | 5.10508 | 10.2 | 8.2 | 9.3 | 1130.6 | 8.5 | 0.337617 |
Band | pI | Loc | % RF | Amplitude | Width | Integr. OD | % Integr. OD | % Lane IntOD | pI | G. Loc | G. Sigma | G. Ampl |
1 | 3.807 | 113 | 9.6 | 0.0392626 | 13 | 0.872099 | 3.4 | 3 | 3.807 | 113.9 | 13 | 0.0378997 |
2 | 4.114 | 158 | 13.4 | 0.562688 | 5 | 5.36219 | 21.2 | 18.6 | 4.114 | 157.9 | 5.3 | 0.569164 |
3 | 4.284 | 183 | 15.5 | 0.421288 | 4 | 3.16627 | 12.5 | 11 | 4.284 | 182.6 | 4.2 | 0.427876 |
4 | 4.434 | 205 | 17.4 | 0.028328 | 5 | 0.222453 | 0.9 | 0.8 | 4.434 | 203.8 | 4.5 | 0.0277602 |
5 | 4.575 | 225 | 19.1 | 0.014928 | 7 | 0.203925 | 0.8 | 0.7 | 4.575 | 222.3 | 7.4 | 0.0155592 |
6 | 4.745 | 245 | 20.8 | 0.0498908 | 6 | 0.56372 | 2.2 | 2 | 4.745 | 246.4 | 6.1 | 0.0520572 |
7 | 4.915 | 265 | 22.5 | 0.0043459 | 6 | 0.111219 | 0.4 | 0.4 | 4.915 | 269.9 | 6 | 0.0104916 |
8 | 5.017 | 277 | 23.5 | 0.0479115 | 4 | 0.284604 | 1.1 | 1 | 5.017 | 276.7 | 3.5 | 0.0455114 |
9 | 5.085 | 285 | 24.2 | 0.0412384 | 3 | 0.245032 | 1 | 0.9 | 5.085 | 285.1 | 3.2 | 0.0433885 |
10 | 5.179 | 296 | 25.1 | 0.03596 | 5 | 0.587512 | 2.3 | 2 | 5.179 | 300.2 | 5.5 | 0.0605537 |
Band | pI | Loc | % RF | Amplitude | Width | Integr. OD | % Integr. OD | % Lane IntOD | pI | G. Loc | G. Sigma | G. Ampl |
11 | 5.252 | 309 | 26.2 | 0.321211 | 5 | 2.42882 | 9.6 | 8.4 | 5.252 | 308.4 | 4.9 | 0.282484 |
12 | 5.306 | 320 | 27.1 | 0.182393 | 5 | 1.54487 | 6.1 | 5.4 | 5.306 | 320.3 | 5.1 | 0.172 |
13 | 5.424 | 344 | 29.2 | 0.0355629 | 5 | 0.287135 | 1.1 | 1 | 5.424 | 343.2 | 4.9 | 0.0333496 |
14 | 5.527 | 365 | 30.9 | 0.00219989 | 4 | 0.0201768 | 0.1 | 0.1 | 5.527 | 364.7 | 3.8 | 0.00301975 |
15 | 5.655 | 391 | 33.1 | 0.202986 | 5 | 1.91616 | 7.6 | 6.7 | 5.655 | 390.9 | 5.2 | 0.209191 |
16 | 5.754 | 411 | 34.8 | 0.0658437 | 4 | 0.3722 | 1.5 | 1.3 | 5.754 | 410.4 | 4.1 | 0.0509305 |
17 | 5.803 | 421 | 35.7 | 0.0537278 | 9 | 0.866489 | 3.4 | 3 | 5.803 | 421.2 | 8.7 | 0.0559968 |
18 | 5.931 | 450 | 38.1 | 0.1094 | 4 | 0.353787 | 1.4 | 1.2 | 5.931 | 447.8 | 4.3 | 0.0468308 |
19 | 5.964 | 458 | 38.8 | 0.0972617 | 12 | 1.9285 | 7.6 | 6.7 | 5.964 | 457.6 | 11.5 | 0.0945712 |
20 | 6.043 | 477 | 40.4 | 0.193823 | 1 | 0.482344 | 1.9 | 1.7 | 6.043 | 477.5 | 1.4 | 0.189503 |
Band | pI | Loc | % RF | Amplitude | Width | Integr. OD | % Integr. OD | % Lane IntOD | pI | G. Loc | G. Sigma | G. Ampl |
21 | 6.121 | 496 | 42 | 0.10261 | 2 | 0.300163 | 1.2 | 1 | 6.121 | 496 | 1.6 | 0.103036 |
22 | 6.159 | 505 | 42.8 | 0.245532 | 1 | 0.638801 | 2.5 | 2.2 | 6.159 | 504.7 | 1.4 | 0.255943 |
23 | 6.196 | 514 | 43.6 | 0.00661662 | 3 | 0.039444 | 0.2 | 0.1 | 6.196 | 513 | 3.1 | 0.00712956 |
24 | 6.262 | 530 | 44.9 | 0.0416078 | 7 | 0.467966 | 1.8 | 1.6 | 6.262 | 530.8 | 6.8 | 0.03907 |
25 | 6.42 | 568 | 48.1 | 0.0103836 | 3 | 0.0659699 | 0.3 | 0.2 | 6.42 | 566.6 | 3.4 | 0.0108133 |
26 | 7.25 | 710 | 60.2 | 0.0323233 | 18 | 1.08594 | 4.3 | 3.8 | 7.25 | 713.5 | 18 | 0.0340381 |
27 | 7.436 | 746 | 63.2 | 0.0624596 | 10 | 0.917436 | 3.6 | 3.2 | 7.436 | 747.7 | 9.7 | 0.0535156 |
Band | pI | Loc | % RF | Amplitude | Width | Integr. OD | % Integr. OD | % Lane IntOD | pI | G. Loc | G. Sigma | G. Ampl |
1 | 3.5 | 74 | 6.3 | 0.226155 | 22 | 7.96329 | 30.7 | 25.1 | 3.5 | 67 | 22.3 | 0.201607 |
2 | 4.55 | 233 | 19.7 | 0.28948 | 5 | 2.24163 | 8.6 | 7.1 | 4.55 | 232.5 | 4.7 | 0.269192 |
3 | 5.2 | 308 | 26.1 | 0.405459 | 4 | 2.74573 | 10.6 | 8.6 | 5.2 | 308.1 | 3.9 | 0.394737 |
4 | 5.85 | 438 | 37.1 | 0.426858 | 3 | 2.23778 | 8.6 | 7 | 5.85 | 438.1 | 3 | 0.417677 |
5 | 6.55 | 601 | 50.9 | 0.365305 | 4 | 2.71658 | 10.5 | 8.5 | 6.55 | 600.3 | 4.4 | 0.351454 |
6 | 6.85 | 636 | 53.9 | 0.0813771 | 4 | 0.501035 | 1.9 | 1.6 | 6.85 | 636.1 | 3.7 | 0.0761395 |
7 | 7.35 | 726 | 61.5 | 0.233125 | 5 | 1.93697 | 7.5 | 6.1 | 7.35 | 725.5 | 4.9 | 0.222796 |
8 | 8.15 | 895 | 75.8 | 0.048874 | 9 | 0.733293 | 2.8 | 2.3 | 8.15 | 894.5 | 8.7 | 0.0477254 |
9 | 8.45 | 962 | 81.5 | 0.0655885 | 6 | 0.674898 | 2.6 | 2.1 | 8.45 | 961.6 | 5.9 | 0.0649015 |
10 | 8.65 | 1004 | 85.1 | 0.0550144 | 6 | 0.506229 | 2 | 1.6 | 8.65 | 1003.5 | 5.6 | 0.0511537 |
Band | pI | Loc | % RF | Amplitude | Width | Integr. OD | % Integr. OD | % Lane IntOD | pI | G. Loc | G. Sigma | G. Ampl |
11 | 9.3 | 1138 | 96.4 | 0.367905 | 6 | 3.67095 | 14.2 | 11.5 | 9.3 | 1136.1 | 6.5 | 0.320738 |
Band | pI | Loc | % RF | Amplitude | Width | Integr. OD | % Integr. OD | % Lane IntOD | pI | G. Loc | G. Sigma | G. Ampl |
1 | 3.5 | 79 | 6.7 | 0.266832 | 11 | 4.35728 | 8.1 | 7.2 | 3.5 | 80.3 | 10.6 | 0.23208 |
2 | 3.85 | 127 | 10.8 | 0.0630145 | 13 | 1.40103 | 2.6 | 2.3 | 3.85 | 126.7 | 13.3 | 0.0593856 |
3 | 4.142 | 167 | 14.2 | 0.624945 | 5 | 5.42757 | 10.1 | 9 | 4.142 | 166.6 | 4.8 | 0.64 |
4 | 4.317 | 191 | 16.2 | 0.478662 | 4 | 3.09775 | 5.7 | 5.1 | 4.317 | 191.1 | 3.7 | 0.476934 |
5 | 4.47 | 212 | 18 | 0.0349213 | 3 | 0.160476 | 0.3 | 0.3 | 4.47 | 210.7 | 2.9 | 0.0313131 |
6 | 4.55 | 223 | 18.9 | 0.247848 | 3 | 1.37357 | 2.5 | 2.3 | 4.55 | 223.6 | 3.3 | 0.23699 |
7 | 4.66 | 236 | 20 | 0.00332369 | 2 | 0.010118 | 0 | 0 | 4.66 | 236.5 | 1.7 | 0.0032625 |
8 | 4.744 | 246 | 20.9 | 0.0103395 | 3 | 0.0518003 | 0.1 | 0.1 | 4.744 | 244.8 | 2.8 | 0.0105261 |
9 | 4.837 | 257 | 21.8 | 0.0964084 | 3 | 0.386706 | 0.7 | 0.6 | 4.837 | 257.5 | 2.9 | 0.0750135 |
10 | 4.921 | 267 | 22.6 | 0.0517916 | 8 | 0.853019 | 1.6 | 1.4 | 4.921 | 266.9 | 8 | 0.0601434 |
Band | pI | Loc | % RF | Amplitude | Width | Integr. OD | % Integr. OD | % Lane IntOD | pI | G. Loc | G. Sigma | G. Ampl |
11 | 5.014 | 278 | 23.6 | 0.112345 | 5 | 0.77826 | 1.4 | 1.3 | 5.014 | 277.8 | 4.5 | 0.0975739 |
12 | 5.09 | 287 | 24.3 | 0.0913199 | 2 | 0.310657 | 0.6 | 0.5 | 5.09 | 287.5 | 2.2 | 0.0803743 |
13 | 5.2 | 300 | 25.4 | 0.207388 | 3 | 1.29414 | 2.4 | 2.1 | 5.2 | 299.4 | 3.4 | 0.213883 |
14 | 5.246 | 310 | 26.3 | 0.543747 | 3 | 3.14116 | 5.8 | 5.2 | 5.246 | 310.3 | 3.2 | 0.551244 |
15 | 5.316 | 325 | 27.6 | 0.357486 | 3 | 1.5535 | 2.9 | 2.6 | 5.316 | 325.6 | 2.8 | 0.318305 |
16 | 5.414 | 346 | 29.3 | 0.0884725 | 3 | 0.471485 | 0.9 | 0.8 | 5.414 | 346 | 3 | 0.0881328 |
17 | 5.511 | 367 | 31.1 | 0.00940321 | 6 | 0.11855 | 0.2 | 0.2 | 5.511 | 367.7 | 6.3 | 0.0106088 |
18 | 5.548 | 375 | 31.8 | 0.0123862 | 1 | 0 | 0 | 0 | 5.548 | 360 | 1 | 0 |
19 | 5.604 | 387 | 32.8 | 0.665466 | 3 | 3.91137 | 7.3 | 6.5 | 5.604 | 387.6 | 3.4 | 0.646403 |
20 | 5.678 | 403 | 34.2 | 0.463935 | 5 | 3.52878 | 6.5 | 5.8 | 5.678 | 402.3 | 5 | 0.401594 |
Band | pI | Loc | % RF | Amplitude | Width | Integr. OD | % Integr. OD | % Lane IntOD | pI | G. Loc | G. Sigma | G. Ampl |
21 | 5.771 | 423 | 35.9 | 0.0867066 | 7 | 1.05409 | 2 | 1.7 | 5.771 | 420.5 | 7.2 | 0.0822301 |
22 | 5.85 | 440 | 37.3 | 0.271512 | 3 | 1.2274 | 2.3 | 2 | 5.85 | 439.8 | 2.5 | 0.271916 |
23 | 5.965 | 466 | 39.5 | 0.0371561 | 3 | 0.231375 | 0.4 | 0.4 | 5.965 | 466.9 | 3.4 | 0.0380928 |
24 | 6.018 | 478 | 40.5 | 0.0156503 | 4 | 0.098728 | 0.2 | 0.2 | 6.018 | 478.3 | 4 | 0.0140815 |
25 | 6.054 | 486 | 41.2 | 0.307781 | 3 | 1.4975 | 2.8 | 2.5 | 6.054 | 486.1 | 2.7 | 0.316267 |
26 | 6.169 | 512 | 43.4 | 0.289869 | 3 | 1.80514 | 3.3 | 3 | 6.169 | 511.3 | 3.4 | 0.295842 |
27 | 6.297 | 541 | 45.9 | 0.0426375 | 8 | 0.543359 | 1 | 0.9 | 6.297 | 541.4 | 8 | 0.038218 |
28 | 6.417 | 568 | 48.2 | 0.041299 | 18 | 1.62724 | 3 | 2.7 | 6.417 | 582.1 | 18 | 0.0511029 |
29 | 6.55 | 598 | 50.7 | 0.206411 | 5 | 1.55915 | 2.9 | 2.6 | 6.55 | 597.1 | 5 | 0.174892 |
30 | 6.85 | 637 | 54 | 0.0845119 | 5 | 0.647678 | 1.2 | 1.1 | 6.85 | 637.8 | 4.7 | 0.0776183 |
Band | pI | Loc | % RF | Amplitude | Width | Integr. OD | % Integr. OD | % Lane IntOD | pI | G. Loc | G. Sigma | G. Ampl |
31 | 7.35 | 728 | 61.7 | 0.269143 | 7 | 3.12117 | 5.8 | 5.1 | 7.35 | 727 | 7.1 | 0.249581 |
32 | 7.463 | 751 | 63.7 | 0.0429303 | 10 | 0.737343 | 1.4 | 1.2 | 7.463 | 750.7 | 9.9 | 0.0420567 |
33 | 8.15 | 891 | 75.6 | 0.261518 | 6 | 2.26908 | 4.2 | 3.7 | 8.15 | 891.2 | 5.6 | 0.228341 |
34 | 8.45 | 954 | 80.9 | 0.0644931 | 6 | 0.632857 | 1.2 | 1 | 8.45 | 954.4 | 6.2 | 0.0572212 |
35 | 8.65 | 1000 | 84.8 | 0.0507905 | 5 | 0.481127 | 0.9 | 0.8 | 8.65 | 999.1 | 5.4 | 0.0505116 |
36 | 9.3 | 1139 | 96.6 | 0.414349 | 7 | 4.13124 | 7.7 | 6.8 | 9.3 | 1136.4 | 6.8 | 0.344519 |
Band | pI | Loc | % RF | Amplitude | Width | Integr. OD | % Integr. OD | % Lane IntOD | pI | G. Loc | G. Sigma | G. Ampl |
1 | 3.747 | 115 | 9.8 | 0.07497 | 8 | 1.08276 | 3.3 | 3 | 3.747 | 115.8 | 8.1 | 0.0750004 |
2 | 4.103 | 164 | 13.9 | 0.551973 | 6 | 5.60743 | 16.9 | 15.7 | 4.103 | 163.8 | 5.6 | 0.56211 |
3 | 4.307 | 192 | 16.3 | 0.316963 | 5 | 3.12073 | 9.4 | 8.7 | 4.307 | 191.7 | 5.4 | 0.325906 |
4 | 4.466 | 214 | 18.2 | 0.0208391 | 6 | 0.197057 | 0.6 | 0.6 | 4.466 | 213.9 | 5.5 | 0.0201172 |
5 | 4.631 | 235 | 19.9 | 0.0112413 | 7 | 0.130454 | 0.4 | 0.4 | 4.631 | 235.2 | 7 | 0.0104992 |
6 | 4.869 | 263 | 22.3 | 0.0315762 | 7 | 0.369736 | 1.1 | 1 | 4.869 | 257.7 | 7.4 | 0.0282534 |
7 | 4.988 | 277 | 23.5 | 0.0146406 | 2 | 0.0488287 | 0.1 | 0.1 | 4.988 | 276.7 | 2.1 | 0.0133766 |
8 | 5.064 | 286 | 24.3 | 0.0161908 | 4 | 0.113936 | 0.3 | 0.3 | 5.064 | 285.6 | 4.2 | 0.0151546 |
9 | 5.264 | 316 | 26.8 | 0.223259 | 10 | 3.53565 | 10.7 | 9.9 | 5.264 | 312.6 | 9.5 | 0.209855 |
10 | 5.327 | 330 | 28 | 0.145468 | 5 | 1.16875 | 3.5 | 3.3 | 5.327 | 329.6 | 5.1 | 0.128639 |
Band | pI | Loc | % RF | Amplitude | Width | Integr. OD | % Integr. OD | % Lane IntOD | pI | G. Loc | G. Sigma | G. Ampl |
11 | 5.409 | 348 | 29.5 | 0.0356881 | 3 | 0.216655 | 0.7 | 0.6 | 5.409 | 347.8 | 3.4 | 0.0356859 |
12 | 5.505 | 369 | 31.3 | 0.0150966 | 9 | 0.350478 | 1.1 | 1 | 5.505 | 374.8 | 9.4 | 0.0210377 |
13 | 5.555 | 380 | 32.2 | 0.0659832 | 3 | 0.537237 | 1.6 | 1.5 | 5.555 | 383.1 | 2.8 | 0.107081 |
14 | 5.6 | 390 | 33.1 | 0.396356 | 6 | 3.94742 | 11.9 | 11 | 5.6 | 389 | 5.9 | 0.377295 |
15 | 5.673 | 406 | 34.4 | 0.283622 | 8 | 3.88272 | 11.7 | 10.8 | 5.673 | 403.9 | 7.5 | 0.290241 |
16 | 5.755 | 424 | 36 | 0.0742989 | 8 | 1.05827 | 3.2 | 3 | 5.755 | 422.9 | 8 | 0.0745158 |
17 | 5.845 | 444 | 37.7 | 0.00750421 | 1 | 0.00984311 | 0 | 0 | 5.845 | 444.3 | 1 | 0.00552897 |
18 | 5.925 | 462 | 39.2 | 0.0147537 | 3 | 0.0798941 | 0.2 | 0.2 | 5.925 | 462 | 3.1 | 0.014567 |
19 | 5.964 | 471 | 39.9 | 0.0342692 | 3 | 0.19387 | 0.6 | 0.5 | 5.964 | 471 | 3.4 | 0.0320541 |
20 | 5.995 | 478 | 40.5 | 0.0221658 | 6 | 0.310464 | 0.9 | 0.9 | 5.995 | 480.3 | 6.4 | 0.0275201 |
Band | pI | Loc | % RF | Amplitude | Width | Integr. OD | % Integr. OD | % Lane IntOD | pI | G. Loc | G. Sigma | G. Ampl |
21 | 6.039 | 488 | 41.4 | 0.262825 | 3 | 1.41271 | 4.3 | 3.9 | 6.039 | 487.7 | 3.2 | 0.249672 |
22 | 6.14 | 511 | 43.3 | 0.283413 | 4 | 1.8417 | 5.6 | 5.1 | 6.14 | 510.7 | 3.7 | 0.282247 |
23 | 6.267 | 540 | 45.8 | 0.0374995 | 6 | 0.32711 | 1 | 0.9 | 6.267 | 540.3 | 6.3 | 0.0293761 |
24 | 6.363 | 562 | 47.7 | 0.0533235 | 16 | 1.43961 | 4.3 | 4 | 6.363 | 563.8 | 16.1 | 0.0504771 |
25 | 6.464 | 585 | 49.6 | 0.0336831 | 11 | 0.510432 | 1.5 | 1.4 | 6.464 | 583.2 | 10.7 | 0.0268871 |
26 | 7.131 | 695 | 58.9 | 0.0299181 | 8 | 0.385424 | 1.2 | 1.1 | 7.131 | 697.2 | 8.3 | 0.0261687 |
27 | 7.317 | 729 | 61.8 | 0.0460139 | 18 | 1.29323 | 3.9 | 3.6 | 7.317 | 731 | 18 | 0.0405356 |
Band | pI | Loc | % RF | Amplitude | Width | Integr. OD | % Integr. OD | % Lane IntOD | pI | G. Loc | G. Sigma | G. Ampl |
1 | 3.5 | 83 | 7 | 0.300114 | 12 | 5.69382 | 24.2 | 18.4 | 3.5 | 84.3 | 11.8 | 0.273286 |
2 | 4.55 | 228 | 19.3 | 0.285799 | 5 | 2.3564 | 10 | 7.6 | 4.55 | 227.4 | 4.8 | 0.275765 |
3 | 5.2 | 304 | 25.8 | 0.367772 | 5 | 2.9727 | 12.6 | 9.6 | 5.2 | 304 | 4.6 | 0.367571 |
4 | 5.85 | 450 | 38.2 | 0.394339 | 3 | 2.28139 | 9.7 | 7.4 | 5.85 | 450 | 3.3 | 0.387927 |
5 | 6.55 | 611 | 51.8 | 0.354021 | 4 | 2.33827 | 9.9 | 7.6 | 6.55 | 610.9 | 4 | 0.330434 |
6 | 6.85 | 650 | 55.1 | 0.143222 | 5 | 1.0525 | 4.5 | 3.4 | 6.85 | 650.1 | 4.6 | 0.130039 |
7 | 7.35 | 742 | 62.9 | 0.177769 | 6 | 1.64688 | 7 | 5.3 | 7.35 | 740.8 | 5.6 | 0.164648 |
8 | 8.15 | 908 | 77 | 0.0447155 | 9 | 0.698033 | 3 | 2.3 | 8.15 | 908.7 | 9.5 | 0.0416562 |
9 | 8.45 | 968 | 82.1 | 0.0704442 | 7 | 0.843544 | 3.6 | 2.7 | 8.45 | 968.5 | 7 | 0.0675365 |
10 | 8.65 | 1008 | 85.5 | 0.0463293 | 5 | 0.43169 | 1.8 | 1.4 | 8.65 | 1007.4 | 5.4 | 0.0448079 |
Band | pI | Loc | % RF | Amplitude | Width | Integr. OD | % Integr. OD | % Lane IntOD | pI | G. Loc | G. Sigma | G. Ampl |
11 | 9.3 | 1139 | 96.6 | 0.350139 | 6 | 3.23016 | 13.7 | 10.4 | 9.3 | 1137.7 | 5.8 | 0.313394 |
a | b | c | d | e | f | g | ||
MK | CO | SP | MK | CO | SP | MK | ||
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Key to Gel Schematic: | MK=pI Marker Lanes, pI bands are in red SP=Specimen Lanes, Specimen bands are in blue CO=Co-migrated Lanes of pI Markers and Specimen (pI bands are in red, Specimen bands are in blue) Note: In co-migrated lanes, some pI Markers and Specimen bands may overlap in experiment. Red pI band is reported when overlapped peak position is equal to nominal pI Marker position. Blue Specimen band is reported when overlapped peak position deviates slightly from nominal pI Marker position. Weak (<2% rel. intensity), Medium (2-6% rel. intensity), and Strong (>6% rel. intensity) band intensities indicated by gel schematic band thickness. Return to Top of Gel Schematic |
DISPLAY GEL LANE LETTER: Lane a DIGITAL REPORT GEL LANE: 1 LANE TYPE: pI Markers ACTUAL GEL LANE: 3 PeakCount: 11 Return to Lane Navigator |
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DISPLAY GEL LANE LETTER: Lane b DIGITAL REPORT GEL LANE: 2 LANE TYPE: Co-Migrated ACTUAL GEL LANE: 4 PeakCount: 34 Return to Lane Navigator |
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DISPLAY GEL LANE LETTER: Lane c DIGITAL REPORT GEL LANE: 3 LANE TYPE: Specimen ACTUAL GEL LANE: 5 PeakCount: 27 Return to Lane Navigator |
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DISPLAY GEL LANE LETTER: Lane d DIGITAL REPORT GEL LANE: 4 LANE TYPE: pI Markers ACTUAL GEL LANE: 6 PeakCount: 11 Return to Lane Navigator |
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DISPLAY GEL LANE LETTER: Lane e DIGITAL REPORT GEL LANE: 5 LANE TYPE: Co-Migrated ACTUAL GEL LANE: 7 PeakCount: 36 Return to Lane Navigator |
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DISPLAY GEL LANE LETTER: Lane f DIGITAL REPORT GEL LANE: 6 LANE TYPE: Specimen ACTUAL GEL LANE: 8 PeakCount: 27 Return to Lane Navigator |
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DISPLAY GEL LANE LETTER: Lane g DIGITAL REPORT GEL LANE: 7 LANE TYPE: pI Markers ACTUAL GEL LANE: 9 PeakCount: 11 Return to Lane Navigator |
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