Fish Image
U.S. Food & Drug Administration
Seafood Products Research Center
Center for Food Safety & Applied Nutrition
Regulatory Fish Encyclopedia:
IEF Gel Lane Banding Pattern Peak Report Data
Gel Image

Peak Report Data from Isoelectric Focusing for a specimen of:
Dolphin  ----  Go to Display Gel Lane Navigator.


FDA Seafood List Scientific Name: Coryphaena hippurus
FDA Seafood List Common Name: Dolphin
FDA Seafood List Market Name: Mahi Mahi
Unique IEF Gel Code: 19981216gel01
Unique Web Code: mhh02Srr
Tissue Aliquot Code: coryhipp J98-002 #1 Gel Analysis Conditions
Gel ImageFillet ImageCalibrated Gel Image
Gel ImageFish ImageCalibrated Gel

DISPLAY GEL LANE NAVIGATOR
RETURN TO TOP OF THIS DOCUMENT
Lane a
pI Markers
Lane b
Specimen
Lane c
pI Markers
Lane d
Co-Migrated
Lane e
Specimen
Lane f
pI Markers
Go to Gel Schematic

 
DISPLAY GEL LANE LETTER: Lane a
DIGITAL REPORT GEL LANE: 2
LANE TYPE: pI Markers
ACTUAL GEL LANE: 2
Go to Gel Schematic
Return to Lane Navigator
BandpILoc% RFAmplitudeWidthIntegr. OD% Integr. OD% Lane IntODpIG. LocG. SigmaG. Ampl
14.55627.60.10250330.5940976.44.84.5561.83.30.102562
25.2155190.12129240.8124168.86.65.2154.940.114287
35.8526031.90.19305730.98902210.78.15.8526030.188517
46.5540349.40.12077871.4501315.711.86.55401.37.30.111683
56.8543853.70.034298130.1921262.11.66.85437.93.20.0339727
67.3550762.10.099076430.5407375.94.47.35506.73.20.0964281
78.1562977.10.13476861.4474515.711.88.15628.56.30.12879
88.4569785.40.1811861.758719.114.38.45696.55.70.175571
98.6574991.80.14415540.853519.378.65748.83.50.136466
109.379597.40.0354049110.58066.34.79.3791.710.60.0309341
PeakCounter: 10
DISPLAY GEL LANE LETTER: Lane b
DIGITAL REPORT GEL LANE: 3
LANE TYPE: Specimen
ACTUAL GEL LANE: 3
Go to Gel Schematic
Return to Lane Navigator
BandpILoc% RFAmplitudeWidthIntegr. OD% Integr. OD% Lane IntODpIG. LocG. SigmaG. Ampl
14.292242.90.052750950.5016550.60.64.29225.75.50.0515198
25.06913416.40.043878760.471750.60.55.069133.75.90.0450192
35.25816219.90.030873830.1675320.20.25.258162.23.30.0282949
45.40418622.80.05045630.2832280.30.35.404186.83.40.0476478
55.56821326.10.031614150.2214720.30.25.568213.150.0252292
65.7524329.80.27256652.115942.62.45.752424.80.248117
75.94527934.20.033489240.2024920.20.25.945278.93.50.0322998
86.20233240.70.039390760.3478730.40.46.202333.45.70.0346492
96.32435743.82.32365312.059814.613.66.324358.52.52.68429
106.411375462.2745429.032261110.26.411374.922.58387
BandpILoc% RFAmplitudeWidthIntegr. OD% Integr. OD% Lane IntODpIG. LocG. SigmaG. Ampl
116.45538447.10.18555372.316442.82.66.4553886.70.194504
126.489391480.71889811.079091.31.26.489390.91.10.548564
136.54840349.40.054890910.09162170.10.16.548403.11.30.0401504
146.63441350.70.1383520.4528090.50.56.634412.620.128752
156.67841851.30.10946120.3866170.50.46.678418.61.90.115335
166.75742752.40.12450430.655580.80.76.757427.32.90.127436
176.83743653.52.317527.341258.98.36.837436.41.72.45489
186.91244654.70.1744831.042991.31.26.912445.73.40.171996
197.13847758.50.056753430.3213380.40.47.138477.23.10.0581596
207.21148759.80.37527721.337571.61.57.211486.620.377226
BandpILoc% RFAmplitudeWidthIntegr. OD% Integr. OD% Lane IntODpIG. LocG. SigmaG. Ampl
217.38351162.72.32876315.254118.517.27.383509.73.32.61431
227.983603742.35884311.429913.912.97.983602.52.62.49299
238.06861675.60.2826773.2760643.78.068616.46.80.271038
248.55172288.60.0333350.2807840.30.38.551720.850.0317662
258.686750920.280253178.3841910.29.58.686755.317.10.276112
269.17778496.20.27345770.383910.50.49.177777.26.80.0318864
279.26479096.90.28587872.957953.63.39.264787.17.10.236184
PeakCounter: 27
DISPLAY GEL LANE LETTER: Lane c
DIGITAL REPORT GEL LANE: 4
LANE TYPE: pI Markers
ACTUAL GEL LANE: 4
Go to Gel Schematic
Return to Lane Navigator
BandpILoc% RFAmplitudeWidthIntegr. OD% Integr. OD% Lane IntODpIG. LocG. SigmaG. Ampl
14.55597.20.12954230.6890045.64.34.5558.93.10.126201
25.215018.40.11388850.9548567.85.95.2150.150.108105
35.8525931.80.26934931.3370510.98.25.852592.90.258087
46.5540449.60.1769971.9738616.112.26.55402.670.158558
56.8543753.60.068996440.439893.62.76.85437.640.062769
67.35505620.17674930.9597277.85.97.35505.23.20.171715
78.1562877.10.15854671.7591214.310.98.15627.96.70.14908
88.4569585.30.19754672.3190318.914.38.45693.96.70.194082
98.6574691.50.18975941.168189.57.28.65745.43.70.178531
109.379096.90.0290703150.6789745.54.29.3785.5150.0254835
PeakCounter: 10
DISPLAY GEL LANE LETTER: Lane d
DIGITAL REPORT GEL LANE: 5
LANE TYPE: Co-Migrated
ACTUAL GEL LANE: 5
Go to Gel Schematic
Return to Lane Navigator
BandpILoc% RFAmplitudeWidthIntegr. OD% Integr. OD% Lane IntODpIG. LocG. SigmaG. Ampl
14.298263.20.0547266100.8757171.31.24.29826.210.20.0484473
24.55597.30.062784130.3016660.40.44.5559.22.70.0626741
35.07812815.80.027201350.2627770.40.45.078128.55.40.027244
45.214417.70.047587970.5187330.80.75.2144.97.20.0404947
55.7523729.20.15379371.741062.52.45.75236.36.70.146264
65.8525431.30.075805230.4246970.60.65.85254.63.20.0742642
76.35536244.62.34445310.98821614.96.3553622.82.21652
86.43537946.70.82271533.713685.456.435379.62.90.718605
96.48138947.90.16873330.7755851.11.16.481388.630.143711
106.51439648.80.33584242.302573.33.16.514396.24.50.288958
BandpILoc% RFAmplitudeWidthIntegr. OD% Integr. OD% Lane IntODpIG. LocG. SigmaG. Ampl
116.73742452.20.065431820.2107350.30.36.737423.91.80.0665977
126.80143153.10.0839346182.384073.53.26.801440.2180.0747272
136.87744054.22.3158126.787529.99.26.877440.21.72.23245
147.167479590.044661520.131220.20.27.167478.320.0366131
157.23448860.10.32939221.202691.71.67.234488.22.20.312157
167.39851162.92.34115313.135419.117.97.398509.72.82.67587
178.01760674.62.3151210.043114.613.78.017605.42.32.48111
188.089617760.32948763.055814.44.28.089617.25.70.300939
198.4569785.80.071995860.720503118.45696.45.90.0684034
208.65747920.230733124.2166.15.78.65748.7120.198504
BandpILoc% RFAmplitudeWidthIntegr. OD% Integr. OD% Lane IntODpIG. LocG. SigmaG. Ampl
218.99277094.80.17553692.272053.33.18.992767.88.70.147599
229.15678196.20.20310982.7093.93.79.156782.38.10.187579
PeakCounter: 22
DISPLAY GEL LANE LETTER: Lane e
DIGITAL REPORT GEL LANE: 6
LANE TYPE: Specimen
ACTUAL GEL LANE: 6
Go to Gel Schematic
Return to Lane Navigator
BandpILoc% RFAmplitudeWidthIntegr. OD% Integr. OD% Lane IntODpIG. LocG. SigmaG. Ampl
14.315273.30.0709558101.07303114.315289.50.0635099
25.12213516.60.076225460.7282890.70.75.122136.25.60.0739964
35.29116119.80.033841660.3000480.30.35.291160.75.60.030468
45.45518923.20.029859740.2211070.20.25.455189.24.50.0278484
55.78924630.30.23348462.122122.11.95.789244.75.70.210294
65.94227633.90.052909130.2692740.30.25.942275.83.20.0472944
76.21533441.10.035939580.4508150.40.46.215336.280.0316786
86.34636244.52.32838312.005311.710.86.3463632.92.36727
96.43138046.72.2700729.614859.48.66.431380.52.42.23222
106.478390480.22591441.563831.51.46.478391.53.90.227579
BandpILoc% RFAmplitudeWidthIntegr. OD% Integr. OD% Lane IntODpIG. LocG. SigmaG. Ampl
116.51139748.81.4205113.30033.236.511396.71.41.32663
126.56540750.10.013890510.08685140.10.16.565408.61.30.0364648
136.66841851.40.14577881.458911.41.36.6684228.40.0984294
146.77943052.90.116662180.6351580.60.66.779438.9180.0199087
156.86744054.12.2990828.1687687.36.867440.11.82.49551
167.15347858.80.073350430.3566270.30.37.1534782.80.0712049
177.2248759.90.559621.769491.71.67.22487.31.80.540087
187.36750762.42.30793313.162712.911.87.3675092.72.70922
197.99560374.22.34346417.61117.215.87.995602.34.42.27413
208.093618760.44339364.1388543.78.093616.95.50.420749
BandpILoc% RFAmplitudeWidthIntegr. OD% Integr. OD% Lane IntODpIG. LocG. SigmaG. Ampl
218.55872388.90.201562155.217325.14.78.558724.314.70.200484
228.70675192.40.526425129.665669.58.78.706750.912.30.444812
238.98777194.80.269566114.452514.448.987771.910.60.23655
249.26779197.30.28441783.868723.83.59.267788.18.20.266703
PeakCounter: 24
DISPLAY GEL LANE LETTER: Lane f
DIGITAL REPORT GEL LANE: 7
LANE TYPE: pI Markers
ACTUAL GEL LANE: 7
Go to Gel Schematic
Return to Lane Navigator
BandpILoc% RFAmplitudeWidthIntegr. OD% Integr. OD% Lane IntODpIG. LocG. SigmaG. Ampl
14.55556.80.098047140.6295035.84.44.5554.83.60.0975152
25.214417.80.09765550.8003797.45.65.2144.34.70.0956747
35.8525631.60.18330530.9627658.96.85.85256.330.178948
46.5540449.80.15637981.8481417.2136.55402.37.50.138359
56.8543653.80.077795340.4857274.53.46.85436.23.90.0694679
67.3550261.90.17406230.9243688.66.57.355023.10.170079
78.1562476.90.14490661.511881410.68.15623.76.20.137995
88.4569085.10.21714762.1148319.614.98.45689.95.70.209911
98.6574491.70.16957141.016499.47.28.65743.63.60.160193
109.379397.80.0299144100.4698534.43.39.3790.110.10.0261416
PeakCounter: 10
Gel Banding Patterns Display in HTML using GIF stacking technique
(Uses Migration Position Normalized Data from above text peak reports)

Return to Lane Navigator    Go to Gel Schematic Key

a b c d e f
MK SP MK CO SP MK
white spacer for gel schematic
pI 4.55 label for gel schematic
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pI 5.20 label for gel schematic
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pI 4.55 label for gel schematic
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pI 5.20 label for gel schematic
white spacer for gel schematic
pI 5.8 label for gel schematic
white spacer for gel schematic
pI 6.55 label for gel schematic
white spacer for gel schematic
pI 6.85 label for gel schematic
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pI 7.35 label for gel schematic
white spacer for gel schematic
pI 8.15 label for gel schematic
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pI 8.45 label for gel schematic
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pI 8.65 label for gel schematic
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Key to Gel Schematic: MK=pI Marker Lanes, pI bands are in red
SP=Specimen Lanes, Specimen bands are in blue
CO=Co-migrated Lanes of pI Markers and Specimen (pI bands are in red, Specimen bands are in blue)
Note:  In co-migrated lanes, some pI Markers and Specimen bands may overlap in experiment.  Red pI
band is reported when overlapped peak position is equal to nominal pI Marker position.  Blue Specimen
band is reported when overlapped peak position deviates slightly from nominal pI Marker position.
Weak (<2% rel. intensity), Medium (2-6% rel. intensity), and Strong (>6% rel. intensity) band intensities indicated by gel schematic band thickness.
Return to Top of Gel Schematic

PeakCount: 103
HTML Forms TextArea Normalized Peak-Position Peak-Area for Copy-Paste events
DISPLAY GEL LANE LETTER: Lane a
DIGITAL REPORT GEL LANE: 2
LANE TYPE: pI Markers
ACTUAL GEL LANE: 2
PeakCount: 10 Return to Lane Navigator
DISPLAY GEL LANE LETTER: Lane b
DIGITAL REPORT GEL LANE: 3
LANE TYPE: Specimen
ACTUAL GEL LANE: 3
PeakCount: 27 Return to Lane Navigator
DISPLAY GEL LANE LETTER: Lane c
DIGITAL REPORT GEL LANE: 4
LANE TYPE: pI Markers
ACTUAL GEL LANE: 4
PeakCount: 10 Return to Lane Navigator
DISPLAY GEL LANE LETTER: Lane d
DIGITAL REPORT GEL LANE: 5
LANE TYPE: Co-Migrated
ACTUAL GEL LANE: 5
PeakCount: 22 Return to Lane Navigator
DISPLAY GEL LANE LETTER: Lane e
DIGITAL REPORT GEL LANE: 6
LANE TYPE: Specimen
ACTUAL GEL LANE: 6
PeakCount: 24 Return to Lane Navigator
DISPLAY GEL LANE LETTER: Lane f
DIGITAL REPORT GEL LANE: 7
LANE TYPE: pI Markers
ACTUAL GEL LANE: 7
PeakCount: 10 Return to Lane Navigator



 RETURN TO TOP THIS DOCUMENT
EXPERIMENTAL CONDITIONS: Gels were scanned at 600 d.p.i. in Adobe Photoshop 3.02 using a Sharp JX-325 Scanner with Film Scanner Attachment. Gels were analyzed for Densitometry, Intregration, and Migration Position Normalization in EXCEL 5.0 Format. *.xls files were imported into Paradox 7.0 to save as *.db files.

 RETURN TO TOP THIS DOCUMENT
Delphi 4.0 Generation of this Isoelectric Focusing (IEF) RFE PAGE 5 Tools in HTML
This HTML is automatically generated from a series of Paradox 7.0 database filesof IEF peak reports is controlled with a main RFEIEF Paradox 7.0 database withDelphi 3.0 front end (see Delphi 2.0 form) and back end for facile addition and modification of the Content of these RFE PAGE 5 HTML tools.
Contact Brad Tenge at btenge@ora.fda.gov for details.


RFE Contacts
A summary list of RFE contact personnel is available at http://www.cfsan.fda.gov/~frf/rfecon.html.

For isoelectric focusing laboratory experiments for fish species identification, you may contact

For isoelectric focusing gel analysis and pattern recognition applications, you may contact

For CFSAN web consultation and posting, you may contact


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Last updated on 2003-FEB-27 by bjt/frf/nln