Seafood Products Research Center Center for Food Safety & Applied Nutrition Regulatory Fish Encyclopedia: IEF Gel Lane Banding Pattern Peak Report Data |
Gel Image | Fish Image | Calibrated Gel |
Lane a pI Markers |
Lane b Specimen |
Lane c pI Markers |
Lane d Co-Migrated |
Lane e Specimen |
Lane f pI Markers |
Go to Gel Schematic |
Band | pI | Loc | % RF | Amplitude | Width | Integr. OD | % Integr. OD | % Lane IntOD | pI | G. Loc | G. Sigma | G. Ampl |
1 | 4.55 | 105 | 12.4 | 0.0405855 | 7 | 0.452926 | 9.3 | 7.5 | 4.55 | 105.2 | 6.9 | 0.0371235 |
2 | 5.2 | 203 | 24 | 0.0360554 | 5 | 0.295231 | 6.1 | 4.9 | 5.2 | 203.2 | 4.9 | 0.0337378 |
3 | 5.85 | 323 | 38.2 | 0.0849837 | 4 | 0.637421 | 13.1 | 10.5 | 5.85 | 323.2 | 4.3 | 0.084573 |
4 | 6.55 | 450 | 53.2 | 0.036457 | 10 | 0.598867 | 12.3 | 9.9 | 6.55 | 448 | 10 | 0.0338203 |
5 | 7.35 | 543 | 64.2 | 0.0195474 | 6 | 0.203112 | 4.2 | 3.3 | 7.35 | 543 | 6.1 | 0.0186671 |
6 | 8.15 | 660 | 78 | 0.053307 | 7 | 0.613771 | 12.6 | 10.1 | 8.15 | 660.3 | 6.8 | 0.0511967 |
7 | 8.45 | 723 | 85.5 | 0.0611684 | 7 | 0.785848 | 16.1 | 13 | 8.45 | 723.1 | 7.4 | 0.0598714 |
8 | 8.65 | 769 | 90.9 | 0.0536689 | 5 | 0.452649 | 9.3 | 7.5 | 8.65 | 768.7 | 4.9 | 0.051878 |
9 | 9.3 | 819 | 96.8 | 0.0711697 | 7 | 0.839788 | 17.2 | 13.8 | 9.3 | 818.9 | 7 | 0.0678076 |
Band | pI | Loc | % RF | Amplitude | Width | Integr. OD | % Integr. OD | % Lane IntOD | pI | G. Loc | G. Sigma | G. Ampl |
1 | 4.073 | 34 | 4 | 0.0670608 | 8 | 0.914065 | 3.5 | 3.1 | 4.073 | 34.5 | 7.7 | 0.0669608 |
2 | 4.804 | 142 | 16.8 | 0.240798 | 7 | 2.89116 | 11.1 | 9.7 | 4.804 | 143.2 | 7.1 | 0.230696 |
3 | 4.953 | 164 | 19.4 | 0.169459 | 9 | 2.56425 | 9.9 | 8.6 | 4.953 | 162.3 | 8.8 | 0.163918 |
4 | 5.318 | 222 | 26.2 | 0.016884 | 4 | 0.119228 | 0.5 | 0.4 | 5.318 | 220.6 | 4.3 | 0.0155188 |
5 | 5.401 | 237 | 28 | 0.417709 | 3 | 2.21941 | 8.5 | 7.4 | 5.401 | 236.8 | 3.3 | 0.378776 |
6 | 5.506 | 256 | 30.3 | 0.0472493 | 17 | 1.53608 | 5.9 | 5.1 | 5.506 | 245 | 17 | 0.0509655 |
7 | 5.643 | 281 | 33.2 | 0.0425903 | 4 | 0.280141 | 1.1 | 0.9 | 5.643 | 281.4 | 4.1 | 0.038114 |
8 | 5.928 | 333 | 39.4 | 0.0189511 | 5 | 0.165031 | 0.6 | 0.6 | 5.928 | 334.5 | 4.9 | 0.0188487 |
9 | 6.046 | 355 | 42 | 0.534509 | 3 | 3.10273 | 11.9 | 10.4 | 6.046 | 354.9 | 3.3 | 0.524433 |
10 | 6.132 | 371 | 43.9 | 0.262491 | 4 | 1.74724 | 6.7 | 5.8 | 6.132 | 370.9 | 4 | 0.248337 |
Band | pI | Loc | % RF | Amplitude | Width | Integr. OD | % Integr. OD | % Lane IntOD | pI | G. Loc | G. Sigma | G. Ampl |
11 | 6.228 | 389 | 46 | 0.137314 | 6 | 1.52624 | 5.9 | 5.1 | 6.228 | 387.2 | 6 | 0.144553 |
12 | 6.496 | 439 | 51.9 | 0.0567924 | 8 | 0.84459 | 3.3 | 2.8 | 6.496 | 441.8 | 8.4 | 0.056523 |
13 | 6.714 | 468 | 55.3 | 0.362047 | 3 | 2.03955 | 7.9 | 6.8 | 6.714 | 468.2 | 3.5 | 0.332885 |
14 | 6.887 | 488 | 57.7 | 0.0355863 | 8 | 0.462886 | 1.8 | 1.5 | 6.887 | 489.5 | 7.9 | 0.0331747 |
15 | 7 | 501 | 59.2 | 0.209917 | 4 | 1.37711 | 5.3 | 4.6 | 7 | 501.1 | 4 | 0.195641 |
16 | 7.129 | 516 | 61 | 0.414084 | 4 | 2.85317 | 11 | 9.6 | 7.129 | 516.1 | 3.9 | 0.412721 |
17 | 7.276 | 533 | 63 | 0.0759968 | 3 | 0.420804 | 1.6 | 1.4 | 7.276 | 533.2 | 3 | 0.0781737 |
18 | 7.448 | 556 | 65.7 | 0.0530191 | 9 | 0.912948 | 3.5 | 3.1 | 7.448 | 554.7 | 9.4 | 0.0545697 |
Band | pI | Loc | % RF | Amplitude | Width | Integr. OD | % Integr. OD | % Lane IntOD | pI | G. Loc | G. Sigma | G. Ampl |
1 | 4.55 | 108 | 12.8 | 0.0552624 | 7 | 0.629959 | 9.9 | 7.9 | 4.55 | 108.2 | 6.7 | 0.0527783 |
2 | 5.2 | 202 | 23.9 | 0.0703863 | 4 | 0.502848 | 7.9 | 6.3 | 5.2 | 201.2 | 4.2 | 0.0681609 |
3 | 5.85 | 318 | 37.6 | 0.119397 | 4 | 0.923573 | 14.6 | 11.6 | 5.85 | 317.9 | 4.4 | 0.118976 |
4 | 6.55 | 452 | 53.4 | 0.058476 | 8 | 0.777906 | 12.3 | 9.7 | 6.55 | 450.6 | 7.7 | 0.0567618 |
5 | 7.35 | 544 | 64.3 | 0.026153 | 6 | 0.26821 | 4.2 | 3.4 | 7.35 | 543.9 | 6.2 | 0.0244592 |
6 | 8.15 | 663 | 78.4 | 0.0617076 | 6 | 0.668194 | 10.5 | 8.4 | 8.15 | 662.2 | 6.4 | 0.0584531 |
7 | 8.45 | 720 | 85.1 | 0.0640588 | 7 | 0.776309 | 12.2 | 9.7 | 8.45 | 720.4 | 7 | 0.0627497 |
8 | 8.65 | 768 | 90.8 | 0.0554606 | 6 | 0.537908 | 8.5 | 6.7 | 8.65 | 767.4 | 5.7 | 0.0529127 |
9 | 9.3 | 820 | 96.9 | 0.115713 | 6 | 1.2525 | 19.8 | 15.7 | 9.3 | 819.8 | 6.4 | 0.111157 |
Band | pI | Loc | % RF | Amplitude | Width | Integr. OD | % Integr. OD | % Lane IntOD | pI | G. Loc | G. Sigma | G. Ampl |
1 | 4.087 | 45 | 5.3 | 0.0191955 | 7 | 0.247067 | 1.6 | 1.5 | 4.087 | 45.9 | 7.4 | 0.0188279 |
2 | 4.55 | 112 | 13.2 | 0.0224421 | 5 | 0.185806 | 1.2 | 1.1 | 4.55 | 111.6 | 5 | 0.0208605 |
3 | 4.778 | 145 | 17.1 | 0.133655 | 6 | 1.42234 | 9.3 | 8.4 | 4.778 | 145.2 | 6.5 | 0.124134 |
4 | 4.903 | 163 | 19.3 | 0.0647431 | 11 | 1.30815 | 8.5 | 7.7 | 4.903 | 161.1 | 11 | 0.067266 |
5 | 5.2 | 206 | 24.3 | 0.0192042 | 4 | 0.141802 | 0.9 | 0.8 | 5.2 | 206 | 4.2 | 0.0189146 |
6 | 5.285 | 221 | 26.1 | 0.0100201 | 3 | 0.0490858 | 0.3 | 0.3 | 5.285 | 220.7 | 2.8 | 0.0097515 |
7 | 5.37 | 236 | 27.9 | 0.24471 | 3 | 1.39834 | 9.1 | 8.2 | 5.37 | 236 | 3.5 | 0.228371 |
8 | 5.471 | 254 | 30 | 0.0247206 | 13 | 0.586763 | 3.8 | 3.5 | 5.471 | 247.3 | 13.1 | 0.0253324 |
9 | 5.601 | 277 | 32.7 | 0.0195597 | 6 | 0.192794 | 1.3 | 1.1 | 5.601 | 275.6 | 5.9 | 0.0183362 |
10 | 5.85 | 321 | 37.9 | 0.0533605 | 4 | 0.374467 | 2.4 | 2.2 | 5.85 | 321.1 | 4 | 0.0531957 |
Band | pI | Loc | % RF | Amplitude | Width | Integr. OD | % Integr. OD | % Lane IntOD | pI | G. Loc | G. Sigma | G. Ampl |
11 | 6.104 | 368 | 43.5 | 0.276411 | 3 | 1.62433 | 10.6 | 9.6 | 6.104 | 367.9 | 3.4 | 0.265884 |
12 | 6.169 | 380 | 44.9 | 0.150409 | 4 | 1.12336 | 7.3 | 6.6 | 6.169 | 379.5 | 4.4 | 0.143767 |
13 | 6.233 | 392 | 46.3 | 0.10301 | 5 | 0.87134 | 5.7 | 5.1 | 6.233 | 392 | 4.7 | 0.10395 |
14 | 6.492 | 440 | 52 | 0.0410764 | 8 | 0.567502 | 3.7 | 3.3 | 6.492 | 440.7 | 8 | 0.0401428 |
15 | 6.68 | 466 | 55.1 | 0.178784 | 3 | 0.745178 | 4.9 | 4.4 | 6.68 | 465.7 | 2.7 | 0.155618 |
16 | 6.833 | 484 | 57.2 | 0.0248934 | 18 | 0.710396 | 4.6 | 4.2 | 6.833 | 474.8 | 18 | 0.022267 |
17 | 6.943 | 497 | 58.7 | 0.0710477 | 3 | 0.323254 | 2.1 | 1.9 | 6.943 | 497.4 | 2.8 | 0.0641271 |
18 | 7.087 | 514 | 60.8 | 0.263593 | 3 | 1.58001 | 10.3 | 9.3 | 7.087 | 513.7 | 3.4 | 0.263223 |
19 | 7.257 | 534 | 63.1 | 0.0343266 | 3 | 0.195212 | 1.3 | 1.1 | 7.257 | 534.3 | 3.3 | 0.0335188 |
20 | 7.35 | 545 | 64.4 | 0.0092179 | 4 | 0.0609975 | 0.4 | 0.4 | 7.35 | 544.1 | 4 | 0.0086647 |
Band | pI | Loc | % RF | Amplitude | Width | Integr. OD | % Integr. OD | % Lane IntOD | pI | G. Loc | G. Sigma | G. Ampl |
21 | 7.482 | 564 | 66.7 | 0.0221673 | 7 | 0.267521 | 1.7 | 1.6 | 7.482 | 563.3 | 7.2 | 0.0208684 |
22 | 8.15 | 660 | 78 | 0.0235168 | 6 | 0.206215 | 1.3 | 1.2 | 8.15 | 660.7 | 5.9 | 0.0198458 |
23 | 8.45 | 725 | 85.7 | 0.0318207 | 7 | 0.372661 | 2.4 | 2.2 | 8.45 | 723.7 | 7 | 0.030218 |
24 | 8.65 | 769 | 90.9 | 0.0255287 | 5 | 0.225053 | 1.5 | 1.3 | 8.65 | 768.1 | 5.1 | 0.0246814 |
25 | 9.3 | 817 | 96.6 | 0.0425352 | 7 | 0.52385 | 3.4 | 3.1 | 9.3 | 817.1 | 7.4 | 0.0397204 |
Band | pI | Loc | % RF | Amplitude | Width | Integr. OD | % Integr. OD | % Lane IntOD | pI | G. Loc | G. Sigma | G. Ampl |
1 | 4.021 | 31 | 3.7 | 0.0892438 | 8 | 1.17163 | 4.4 | 3.9 | 4.021 | 31.6 | 7.7 | 0.0860321 |
2 | 4.758 | 141 | 16.6 | 0.265086 | 6 | 2.77625 | 10.5 | 9.2 | 4.758 | 140.9 | 6.2 | 0.250754 |
3 | 4.898 | 162 | 19.1 | 0.121678 | 11 | 2.35111 | 8.9 | 7.8 | 4.898 | 159.7 | 11.1 | 0.119938 |
4 | 5.29 | 223 | 26.3 | 0.0099817 | 4 | 0.0718471 | 0.3 | 0.2 | 5.29 | 222.7 | 4.2 | 0.0096304 |
5 | 5.379 | 239 | 28.2 | 0.415468 | 4 | 2.69329 | 10.2 | 8.9 | 5.379 | 238.7 | 3.9 | 0.390958 |
6 | 5.469 | 255 | 30.1 | 0.0734699 | 13 | 1.52564 | 5.8 | 5 | 5.469 | 251.8 | 12.7 | 0.0675948 |
7 | 5.587 | 276 | 32.5 | 0.065795 | 6 | 0.614072 | 2.3 | 2 | 5.587 | 276.3 | 5.8 | 0.0598474 |
8 | 5.986 | 348 | 41 | 0.0212616 | 3 | 0.127962 | 0.5 | 0.4 | 5.986 | 347.6 | 3.4 | 0.0215088 |
9 | 6.063 | 362 | 42.7 | 0.446317 | 3 | 2.72744 | 10.4 | 9 | 6.063 | 361.9 | 3.5 | 0.44016 |
10 | 6.139 | 376 | 44.3 | 0.280067 | 4 | 1.91522 | 7.3 | 6.3 | 6.139 | 375.4 | 4 | 0.270985 |
Band | pI | Loc | % RF | Amplitude | Width | Integr. OD | % Integr. OD | % Lane IntOD | pI | G. Loc | G. Sigma | G. Ampl |
11 | 6.21 | 389 | 45.9 | 0.175373 | 5 | 1.56337 | 5.9 | 5.2 | 6.21 | 388.3 | 4.8 | 0.183268 |
12 | 6.275 | 401 | 47.3 | 0.0060515 | 1 | 0.004832 | 0 | 0 | 6.275 | 402 | 1.4 | 0.0019998 |
13 | 6.466 | 436 | 51.4 | 0.0595059 | 6 | 0.671394 | 2.5 | 2.2 | 6.466 | 436.3 | 6.4 | 0.0589331 |
14 | 6.599 | 457 | 53.9 | 0.0842163 | 3 | 0.338596 | 1.3 | 1.1 | 6.599 | 456.9 | 2.7 | 0.0708809 |
15 | 6.652 | 463 | 54.6 | 0.402161 | 2 | 1.6089 | 6.1 | 5.3 | 6.652 | 463 | 2.4 | 0.375171 |
16 | 6.835 | 484 | 57.1 | 0.0423218 | 20 | 1.33242 | 5.1 | 4.4 | 6.835 | 478 | 19.5 | 0.0385379 |
17 | 6.949 | 497 | 58.6 | 0.197815 | 3 | 1.05488 | 4 | 3.5 | 6.949 | 496.7 | 3.3 | 0.179667 |
18 | 7.097 | 514 | 60.6 | 0.399606 | 4 | 2.51463 | 9.5 | 8.3 | 7.097 | 514.5 | 3.6 | 0.399049 |
19 | 7.271 | 534 | 63 | 0.0797307 | 3 | 0.432199 | 1.6 | 1.4 | 7.271 | 533.5 | 3 | 0.0804854 |
20 | 7.459 | 559 | 65.9 | 0.0441017 | 11 | 0.84756 | 3.2 | 2.8 | 7.459 | 556.5 | 10.8 | 0.0442254 |
Band | pI | Loc | % RF | Amplitude | Width | Integr. OD | % Integr. OD | % Lane IntOD | pI | G. Loc | G. Sigma | G. Ampl |
1 | 4.55 | 106 | 12.5 | 0.0659392 | 7 | 0.776319 | 9.1 | 7.6 | 4.55 | 106.5 | 7.2 | 0.0604681 |
2 | 5.2 | 206 | 24.3 | 0.0913815 | 4 | 0.653058 | 7.7 | 6.4 | 5.2 | 205.7 | 4.2 | 0.088341 |
3 | 5.85 | 323 | 38.2 | 0.156959 | 4 | 1.18696 | 14 | 11.6 | 5.85 | 322.8 | 4.3 | 0.154434 |
4 | 6.55 | 450 | 53.2 | 0.0638595 | 8 | 0.923877 | 10.9 | 9 | 6.55 | 448.5 | 8.1 | 0.064229 |
5 | 7.35 | 539 | 63.7 | 0.0446071 | 6 | 0.430948 | 5.1 | 4.2 | 7.35 | 539.2 | 5.5 | 0.0439266 |
6 | 8.15 | 659 | 77.9 | 0.0732513 | 7 | 0.89954 | 10.6 | 8.8 | 8.15 | 659.4 | 7.2 | 0.0700352 |
7 | 8.45 | 719 | 85 | 0.0790386 | 9 | 1.28211 | 15.1 | 12.5 | 8.45 | 717.9 | 9.4 | 0.0769278 |
8 | 8.65 | 761 | 90 | 0.0830607 | 5 | 0.73926 | 8.7 | 7.2 | 8.65 | 760.4 | 5.2 | 0.0807518 |
9 | 9.3 | 814 | 96.2 | 0.138214 | 7 | 1.59737 | 18.8 | 15.6 | 9.3 | 813.4 | 6.8 | 0.131591 |
a | b | c | d | e | f | ||
MK | SP | MK | CO | SP | MK | ||
Key to Gel Schematic: | MK=pI Marker Lanes, pI bands are in red SP=Specimen Lanes, Specimen bands are in blue CO=Co-migrated Lanes of pI Markers and Specimen (pI bands are in red, Specimen bands are in blue) Note: In co-migrated lanes, some pI Markers and Specimen bands may overlap in experiment. Red pI band is reported when overlapped peak position is equal to nominal pI Marker position. Blue Specimen band is reported when overlapped peak position deviates slightly from nominal pI Marker position. Weak (<2% rel. intensity), Medium (2-6% rel. intensity), and Strong (>6% rel. intensity) band intensities indicated by gel schematic band thickness. Return to Top of Gel Schematic |
DISPLAY GEL LANE LETTER: Lane a DIGITAL REPORT GEL LANE: 2 LANE TYPE: pI Markers ACTUAL GEL LANE: 2 PeakCount: 9 Return to Lane Navigator |
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DISPLAY GEL LANE LETTER: Lane b DIGITAL REPORT GEL LANE: 3 LANE TYPE: Specimen ACTUAL GEL LANE: 3 PeakCount: 18 Return to Lane Navigator |
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DISPLAY GEL LANE LETTER: Lane c DIGITAL REPORT GEL LANE: 4 LANE TYPE: pI Markers ACTUAL GEL LANE: 4 PeakCount: 9 Return to Lane Navigator |
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DISPLAY GEL LANE LETTER: Lane d DIGITAL REPORT GEL LANE: 5 LANE TYPE: Co-Migrated ACTUAL GEL LANE: 5 PeakCount: 25 Return to Lane Navigator |
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DISPLAY GEL LANE LETTER: Lane e DIGITAL REPORT GEL LANE: 6 LANE TYPE: Specimen ACTUAL GEL LANE: 6 PeakCount: 20 Return to Lane Navigator |
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DISPLAY GEL LANE LETTER: Lane f DIGITAL REPORT GEL LANE: 7 LANE TYPE: pI Markers ACTUAL GEL LANE: 7 PeakCount: 9 Return to Lane Navigator |
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