Fish Image
U.S. Food & Drug Administration
Seafood Products Research Center
Center for Food Safety & Applied Nutrition
Regulatory Fish Encyclopedia:
IEF Gel Lane Banding Pattern Peak Report Data
Gel Image

Peak Report Data from Isoelectric Focusing for a specimen of:
Kawakawa  ----  Go to Display Gel Lane Navigator.


FDA Seafood List Scientific Name: Euthynnus affinis
FDA Seafood List Common Name: Kawakawa
FDA Seafood List Market Name: Tuna
Unique IEF Gel Code: 19970501gel03
Unique Web Code: kwh02Srr
Tissue Aliquot Code: euthaffi A97-003 #05p Gel Analysis Conditions
Gel ImageFish ImageCalibrated Gel Image
Gel ImageFish ImageCalibrated Gel

DISPLAY GEL LANE NAVIGATOR
RETURN TO TOP OF THIS DOCUMENT
Lane a
pI Markers
Lane b
Specimen
Lane c
pI Markers
Lane d
Co-Migrated
Lane e
Specimen
Lane f
pI Markers
Go to Gel Schematic

 
DISPLAY GEL LANE LETTER: Lane a
DIGITAL REPORT GEL LANE: 2
LANE TYPE: pI Markers
ACTUAL GEL LANE: 2
Go to Gel Schematic
Return to Lane Navigator
BandpILoc% RFAmplitudeWidthIntegr. OD% Integr. OD% Lane IntODpIG. LocG. SigmaG. Ampl
14.55131.70.062791130.3401914.63.44.5513.730.0629453
25.211014.10.10510550.84230911.38.35.2110.24.70.102117
35.8520225.90.067736140.4812476.54.85.852024.30.0638131
46.5534744.40.11724371.2130616.3126.55345.86.60.104162
56.8537948.50.044526960.4095475.54.16.85379.45.70.0403386
67.3545257.90.089471440.6339278.56.37.3545240.0901745
78.1557473.50.10728671.3049917.512.98.15575.170.104488
88.4564782.80.079880560.8772711.88.78.45646.26.40.0779014
98.65703900.087127140.6515828.86.48.65702.24.30.0848424
109.376297.60.074244250.6861679.26.89.3761.75.40.0711885
PeakCounter: 10
DISPLAY GEL LANE LETTER: Lane b
DIGITAL REPORT GEL LANE: 3
LANE TYPE: Specimen
ACTUAL GEL LANE: 3
Go to Gel Schematic
Return to Lane Navigator
BandpILoc% RFAmplitudeWidthIntegr. OD% Integr. OD% Lane IntODpIG. LocG. SigmaG. Ampl
14.875597.60.0300864100.4601692.324.87562.910.20.0254173
25.82119825.40.13397840.827854.23.65.821198.23.50.133099
36.34830639.20.012726540.0775480.40.36.348304.73.50.0123265
46.45932942.10.071191290.8544234.33.76.459329.28.70.0553414
56.53634544.20.16343241.006245.14.36.536344.83.70.154755
66.61335545.50.37379432.2114811.19.66.6133553.30.380087
76.81937848.40.17267741.256666.35.46.819378.44.40.159644
86.91139049.90.08529550.7355863.73.26.911390.35.10.081047
96.97539951.10.031571540.1274980.60.66.9753983.60.0198207
107.06841252.80.45182532.3997312.110.47.068411.730.454707
BandpILoc% RFAmplitudeWidthIntegr. OD% Integr. OD% Lane IntODpIG. LocG. SigmaG. Ampl
117.20443155.20.040628290.5340932.72.37.204431.98.50.0353945
127.31144657.10.10402140.6761843.42.97.3114454.20.0897797
137.36453580.089883950.7834343.93.47.3645450.088815
147.49647460.70.16468141.012435.14.47.496473.93.60.160833
157.612492630.35029742.2259511.29.67.612491.93.60.344968
168.33861879.10.073251781.034535.24.58.338617.38.30.0701748
178.38262880.40.17226641.193965.28.382628.74.20.160973
188.45264482.50.015129330.08763480.40.48.452644.93.30.0151945
198.55867486.30.0556731141.283666.55.58.558670.7140.051641
208.73570890.70.000641910008.73570410
BandpILoc% RFAmplitudeWidthIntegr. OD% Integr. OD% Lane IntODpIG. LocG. SigmaG. Ampl
219.30576297.60.080795681.058855.34.69.305761.37.80.0766492
PeakCounter: 21
DISPLAY GEL LANE LETTER: Lane c
DIGITAL REPORT GEL LANE: 4
LANE TYPE: pI Markers
ACTUAL GEL LANE: 4
Go to Gel Schematic
Return to Lane Navigator
BandpILoc% RFAmplitudeWidthIntegr. OD% Integr. OD% Lane IntODpIG. LocG. SigmaG. Ampl
14.55182.30.037263640.2823775.64.14.5518.44.30.0369542
25.29512.20.043399460.4087358.15.95.295.95.60.0414412
35.85203260.04260140.3087036.14.45.85202.240.0431413
46.5534944.70.068140170.75643815.110.96.55348.56.70.0634205
56.8538449.20.026129150.2232474.43.26.85384.65.40.023507
67.3545157.70.049268140.3191756.44.67.35450.73.80.0473335
78.1557673.80.086134471.0121820.214.68.15576.36.80.0836589
88.4564081.90.085601270.9917619.714.38.456406.90.0807025
98.6569789.20.051627250.4595629.16.68.65697.45.20.0501321
109.376197.40.036145340.2608585.23.89.3760.54.30.0343414
PeakCounter: 10
DISPLAY GEL LANE LETTER: Lane d
DIGITAL REPORT GEL LANE: 5
LANE TYPE: Co-Migrated
ACTUAL GEL LANE: 5
Go to Gel Schematic
Return to Lane Navigator
BandpILoc% RFAmplitudeWidthIntegr. OD% Integr. OD% Lane IntODpIG. LocG. SigmaG. Ampl
14.55212.70.034011540.2181931.41.24.5521.43.70.0331335
24.864627.90.0201555110.355162.224.8646410.70.0187805
35.210613.60.052767450.4345022.72.55.2105.84.90.0501131
45.8520225.90.076482550.5791633.73.35.85202.14.60.0707749
56.36131139.80.007689340.0538130.30.36.361311.93.60.0083639
66.43132641.70.006783920.02744560.20.26.431325.62.30.0066333
76.49233943.40.042163260.350752.226.492339.76.20.0319627
86.57335445.30.13467240.8515995.44.86.573353.93.70.129458
96.64136246.40.31807631.7095210.89.66.641361.930.322323
106.80438148.80.17815941.164447.46.66.8043813.90.168242
BandpILoc% RFAmplitudeWidthIntegr. OD% Integr. OD% Lane IntODpIG. LocG. SigmaG. Ampl
116.88639150.10.11118350.8616545.54.96.886389.54.70.104137
126.939398510.037777870.3919832.52.26.939397.170.0316162
137.01640852.20.43957132.4025915.213.67.016408.130.44732
147.13842454.30.023635340.1710231.117.138423.340.0239222
157.26844156.50.082393130.369042.32.17.268440.62.70.0761333
167.3344957.50.10888850.9025135.75.17.33449.54.60.110905
177.4947360.60.1459140.9186145.85.27.49472.83.80.13466
187.614492630.3074541.958912.411.17.614492.23.80.294603
198.1557473.50.048039350.4258892.72.48.155745.40.044769
208.31461078.10.020409570.25461.61.48.314611.37.30.0196952
BandpILoc% RFAmplitudeWidthIntegr. OD% Integr. OD% Lane IntODpIG. LocG. SigmaG. Ampl
218.36862279.60.080944840.5836043.73.38.368622.34.10.0796772
228.4564081.90.021256450.2165961.41.28.45640.15.50.0222537
238.5566985.70.008964460.1017050.60.68.55667.15.90.0097026
248.6569889.40.022847640.145340.90.88.656983.70.022244
259.375796.90.028249170.3581982.329.3756.57.20.0281351
PeakCounter: 25
DISPLAY GEL LANE LETTER: Lane e
DIGITAL REPORT GEL LANE: 6
LANE TYPE: Specimen
ACTUAL GEL LANE: 6
Go to Gel Schematic
Return to Lane Navigator
BandpILoc% RFAmplitudeWidthIntegr. OD% Integr. OD% Lane IntODpIG. LocG. SigmaG. Ampl
14.98178100.041072280.51701721.74.98174.48.10.036136
25.83820826.60.11135440.7688642.92.55.838207.83.90.110995
36.34231139.80.011214830.07121870.30.26.342310.73.10.0128489
46.39632241.20.009550790.1925390.70.66.396329.18.90.0122007
56.464336430.045905430.1766560.70.66.464336.12.80.0361846
66.54235245.10.15328630.8995293.42.96.5423523.50.146391
76.62136246.40.33394831.978457.66.46.621362.23.30.339432
86.801383490.17425541.136934.33.76.801383.140.158734
96.89239450.40.094817581.263234.84.16.892394.58.20.0870997
107.04141352.90.43824232.6303310.18.57.041413.13.40.435145
BandpILoc% RFAmplitudeWidthIntegr. OD% Integr. OD% Lane IntODpIG. LocG. SigmaG. Ampl
117.17543055.10.037653490.5564292.11.87.175432.38.80.0358223
127.27744356.70.11232340.6714082.62.27.277442.340.0937333
137.34745257.90.071970650.7258412.82.37.347451.15.20.0781432
147.48547360.60.1691431.011653.93.37.485473.43.40.169008
157.62849563.40.34184442.340218.97.57.628494.740.327675
168.31561378.50.0983157101.620566.25.28.3156149.70.0944202
178.3862880.40.2262862.38399.17.78.38628.16.50.208494
188.4564482.50.093301450.3956471.51.38.45645.24.90.0460221
198.5767586.40.144752184.458741714.48.57669.9180.139757
208.729703900.0726585171.610866.25.28.729700.616.60.0546829
BandpILoc% RFAmplitudeWidthIntegr. OD% Integr. OD% Lane IntODpIG. LocG. SigmaG. Ampl
219.30575897.10.056809880.7550692.92.49.305756.67.80.0543994
PeakCounter: 21
DISPLAY GEL LANE LETTER: Lane f
DIGITAL REPORT GEL LANE: 7
LANE TYPE: pI Markers
ACTUAL GEL LANE: 7
Go to Gel Schematic
Return to Lane Navigator
BandpILoc% RFAmplitudeWidthIntegr. OD% Integr. OD% Lane IntODpIG. LocG. SigmaG. Ampl
14.55273.50.047461340.3446184.73.64.5527.44.10.0475469
25.210513.40.074873150.6770159.27.15.21065.40.0711637
35.8521827.90.042439550.37000253.95.85217.55.10.0409576
46.5535645.60.13135651.1343515.5126.55355.95.30.121119
56.8539150.10.046651350.3889135.34.16.85391.450.0436107
67.35453580.085158140.6195748.46.57.35452.74.10.0850359
78.1557673.80.083326991.2422216.913.18.15574.88.60.0810262
88.45648830.097117271.2511517.113.28.45647.37.40.0951359
98.6569388.70.065877570.74110210.17.88.65692.56.80.061446
109.375897.10.0223329120.5657867.769.375011.80.0271144
PeakCounter: 10
Gel Banding Patterns Display in HTML using GIF stacking technique
(Uses Migration Position Normalized Data from above text peak reports)

Return to Lane Navigator    Go to Gel Schematic Key

a b c d e f
MK SP MK CO SP MK
white spacer for gel schematic
pI 4.55 label for gel schematic
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pI 5.20 label for gel schematic
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pI 5.8 label for gel schematic
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pI 4.55 label for gel schematic
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pI 5.20 label for gel schematic
white spacer for gel schematic
pI 5.8 label for gel schematic
white spacer for gel schematic
pI 6.55 label for gel schematic
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pI 6.85 label for gel schematic
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pI 7.35 label for gel schematic
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pI 8.15 label for gel schematic
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pI 8.45 label for gel schematic
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Key to Gel Schematic: MK=pI Marker Lanes, pI bands are in red
SP=Specimen Lanes, Specimen bands are in blue
CO=Co-migrated Lanes of pI Markers and Specimen (pI bands are in red, Specimen bands are in blue)
Note:  In co-migrated lanes, some pI Markers and Specimen bands may overlap in experiment.  Red pI
band is reported when overlapped peak position is equal to nominal pI Marker position.  Blue Specimen
band is reported when overlapped peak position deviates slightly from nominal pI Marker position.
Weak (<2% rel. intensity), Medium (2-6% rel. intensity), and Strong (>6% rel. intensity) band intensities indicated by gel schematic band thickness.
Return to Top of Gel Schematic

PeakCount: 97
HTML Forms TextArea Normalized Peak-Position Peak-Area for Copy-Paste events
DISPLAY GEL LANE LETTER: Lane a
DIGITAL REPORT GEL LANE: 2
LANE TYPE: pI Markers
ACTUAL GEL LANE: 2
PeakCount: 10 Return to Lane Navigator
DISPLAY GEL LANE LETTER: Lane b
DIGITAL REPORT GEL LANE: 3
LANE TYPE: Specimen
ACTUAL GEL LANE: 3
PeakCount: 21 Return to Lane Navigator
DISPLAY GEL LANE LETTER: Lane c
DIGITAL REPORT GEL LANE: 4
LANE TYPE: pI Markers
ACTUAL GEL LANE: 4
PeakCount: 10 Return to Lane Navigator
DISPLAY GEL LANE LETTER: Lane d
DIGITAL REPORT GEL LANE: 5
LANE TYPE: Co-Migrated
ACTUAL GEL LANE: 5
PeakCount: 25 Return to Lane Navigator
DISPLAY GEL LANE LETTER: Lane e
DIGITAL REPORT GEL LANE: 6
LANE TYPE: Specimen
ACTUAL GEL LANE: 6
PeakCount: 21 Return to Lane Navigator
DISPLAY GEL LANE LETTER: Lane f
DIGITAL REPORT GEL LANE: 7
LANE TYPE: pI Markers
ACTUAL GEL LANE: 7
PeakCount: 10 Return to Lane Navigator



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EXPERIMENTAL CONDITIONS: Gels were scanned at 600 d.p.i. in Adobe Photoshop 3.02 using a Sharp JX-325 Scanner with Film Scanner Attachment. Gels were analyzed for Densitometry, Intregration, and Migration Position Normalization in EXCEL 5.0 Format. *.xls files were imported into Paradox 7.0 to save as *.db files.

 RETURN TO TOP THIS DOCUMENT
Delphi 4.0 Generation of this Isoelectric Focusing (IEF) RFE PAGE 5 Tools in HTML
This HTML is automatically generated from a series of Paradox 7.0 database filesof IEF peak reports is controlled with a main RFEIEF Paradox 7.0 database withDelphi 3.0 front end (see Delphi 2.0 form) and back end for facile addition and modification of the Content of these RFE PAGE 5 HTML tools.
Contact Brad Tenge at btenge@ora.fda.gov for details.


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A summary list of RFE contact personnel is available at http://www.cfsan.fda.gov/~frf/rfecon.html.

For isoelectric focusing laboratory experiments for fish species identification, you may contact

For isoelectric focusing gel analysis and pattern recognition applications, you may contact

For CFSAN web consultation and posting, you may contact


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Last updated on 2003-FEB-27 by bjt/frf/nln