Fish Image
U.S. Food & Drug Administration
Seafood Products Research Center
Center for Food Safety & Applied Nutrition
Regulatory Fish Encyclopedia:
IEF Gel Lane Banding Pattern Peak Report Data
Gel Image

Peak Report Data from Isoelectric Focusing for a specimen of:
Coho Salmon  ----  Go to Display Gel Lane Navigator.


FDA Seafood List Scientific Name: Oncorhynchus kisutch
FDA Seafood List Common Name: Coho Salmon
FDA Seafood List Market Name: Salmon, Coho (Silver, Medium Red)
Unique IEF Gel Code: 19940830gel05
Unique Web Code: coh02Srr
Tissue Aliquot Code: oncokisu H94-003 #04a Gel Analysis Conditions
Gel ImageFish ImageCalibrated Gel Image
Gel ImageFish ImageCalibrated Gel

DISPLAY GEL LANE NAVIGATOR
RETURN TO TOP OF THIS DOCUMENT
Lane a
pI Markers
Lane b
Specimen
Lane c
pI Markers
Lane d
Co-Migrated
Lane e
Specimen
Lane f
pI Markers
Go to Gel Schematic

 
DISPLAY GEL LANE LETTER: Lane a
DIGITAL REPORT GEL LANE: 2
LANE TYPE: pI Markers
ACTUAL GEL LANE: 2
Go to Gel Schematic
Return to Lane Navigator
BandpILoc% RFAmplitudeWidthIntegr. OD% Integr. OD% Lane IntODpIG. LocG. SigmaG. Ampl
14.55374.80.076872260.7258327.86.14.5536.85.60.0736189
25.213417.30.12781650.99057810.68.35.2133.74.50.123522
35.8524531.60.16528141.2808513.810.75.85245.44.50.161664
46.5537247.90.097341381.305021410.96.55371.180.0921145
56.8540752.40.020363660.19004421.66.85407.15.60.0191261
67.35473610.061689260.6654377.15.67.35472.86.30.0600522
78.1559476.50.090217781.181612.79.98.15593.57.60.0872887
88.4565784.70.084551781.1344612.29.58.45656.17.60.0837985
98.6570390.60.063542840.4842545.24.18.65702.94.40.0622427
109.374796.30.11636171.354514.511.39.3746.57.30.104568
PeakCounter: 10
DISPLAY GEL LANE LETTER: Lane b
DIGITAL REPORT GEL LANE: 3
LANE TYPE: Specimen
ACTUAL GEL LANE: 3
Go to Gel Schematic
Return to Lane Navigator
BandpILoc% RFAmplitudeWidthIntegr. OD% Integr. OD% Lane IntODpIG. LocG. SigmaG. Ampl
14.455273.50.007055970.07543720.20.14.45529.67.10.0060229
24.66567.20.027442950.2480020.50.54.6656.35.20.027135
34.8868811.30.071310170.7937071.71.54.88687.57.20.0621306
44.977101130.13225461.397162.92.64.977101.26.20.127405
55.38816521.30.0726368182.188574.64.15.388164.918.50.0668491
65.56719625.30.13327892.054994.33.95.567194.29.30.124336
75.83824331.30.021735760.2050110.40.45.838244.36.30.0184985
86.14630038.70.29506131.682773.53.26.146300.13.40.282514
96.21631340.30.20869951.62093.43.16.216312.34.80.188628
106.286326420.090390561.021432.11.96.286325.16.50.0892423
BandpILoc% RFAmplitudeWidthIntegr. OD% Integr. OD% Lane IntODpIG. LocG. SigmaG. Ampl
116.37834344.20.58293233.423177.26.46.378343.33.30.580648
126.46936046.40.35825742.293264.84.36.469359.33.70.346756
136.52837147.80.16750351.4115532.76.528370.14.70.16801
146.63738549.60.33740931.689623.53.26.637384.82.80.334817
156.68939150.40.52258132.571715.44.86.689390.72.70.532789
166.78540251.80.16106130.801161.71.56.785402.32.80.163441
177.1344557.30.25430531.318572.82.57.13445.52.90.255161
187.31146860.30.28404941.956574.13.77.311468.340.278442
197.38347861.60.39018832.309494.84.47.383477.53.40.378722
207.48349363.50.45017643.207946.767.483492.94.10.440697
BandpILoc% RFAmplitudeWidthIntegr. OD% Integr. OD% Lane IntODpIG. LocG. SigmaG. Ampl
217.6952467.50.2174331.052472.227.69523.72.80.212175
227.7553368.70.32716752.682235.65.17.75532.94.70.322215
238.74370891.20.212561155.5541711.610.58.743708.715.10.207453
248.91472092.80.24019240.4722510.98.914720.44.40.0602821
259.07173194.20.268656125.6792811.910.79.071735120.266111
PeakCounter: 25
DISPLAY GEL LANE LETTER: Lane c
DIGITAL REPORT GEL LANE: 4
LANE TYPE: pI Markers
ACTUAL GEL LANE: 4
Go to Gel Schematic
Return to Lane Navigator
BandpILoc% RFAmplitudeWidthIntegr. OD% Integr. OD% Lane IntODpIG. LocG. SigmaG. Ampl
14.55435.50.07633150.6571547.25.74.5542.950.073808
25.213016.80.14707351.1980413.210.45.2130.34.90.138566
35.8524431.50.14341341.0131211.28.85.85244.140.142841
46.5537748.60.11225371.3223514.611.46.55376.170.105926
56.85411530.023718160.2595772.92.26.85410.66.50.0225452
67.3547260.90.060893160.6252586.95.47.35471.35.90.0594377
78.1559176.30.091527771.177361310.28.15590.97.30.0906894
88.45651840.064936670.8670159.57.58.45652.96.90.0709272
98.6569990.20.053018550.472925.24.18.65698.75.20.0516477
109.374696.30.13354271.4915716.412.99.3745.37.20.117254
PeakCounter: 10
DISPLAY GEL LANE LETTER: Lane d
DIGITAL REPORT GEL LANE: 5
LANE TYPE: Co-Migrated
ACTUAL GEL LANE: 5
Go to Gel Schematic
Return to Lane Navigator
BandpILoc% RFAmplitudeWidthIntegr. OD% Integr. OD% Lane IntODpIG. LocG. SigmaG. Ampl
14.55445.70.032010650.2863240.60.64.5543.35.20.031106
24.6195470.015291750.1194350.30.24.61955.24.80.0139969
34.88993120.034368160.3383990.80.74.88993.96.20.0308052
44.97210513.50.068043160.7516421.71.54.972105.36.20.0679312
55.213817.80.033258560.3324140.80.75.2138.65.60.0336342
65.36816721.50.035423960.3473840.80.75.368166.75.80.0340246
75.525194250.099292891.570933.63.25.525191.88.60.102793
85.8525032.30.052695960.534671.21.15.85249.65.80.0519296
96.12329838.50.16080661.541893.53.26.123298.35.70.152161
106.21431440.50.13474561.346473.12.86.214313.35.90.127896
BandpILoc% RFAmplitudeWidthIntegr. OD% Integr. OD% Lane IntODpIG. LocG. SigmaG. Ampl
116.29432842.30.053275370.6694411.51.46.294326.26.60.0572591
126.39134544.50.46297532.834096.45.86.3913453.50.456883
136.48236146.60.25689941.888284.33.96.482361.24.30.246794
146.5537348.10.12037451.059622.42.26.55372.44.80.124854
156.6538649.80.20082341.506463.43.16.65387.53.60.233175
166.70439350.70.35106431.923624.43.96.704393.33.20.335435
176.80440652.40.080963330.48381.116.804405.73.30.0835373
187.17545158.20.15417641.000822.327.175450.43.60.154712
197.3547260.90.23943541.944174.447.35472.94.40.247239
207.41148162.10.28167741.913654.33.97.411480.94.10.266173
BandpILoc% RFAmplitudeWidthIntegr. OD% Integr. OD% Lane IntODpIG. LocG. SigmaG. Ampl
217.50649563.90.3447352.82536.45.87.506495.34.70.34027
227.69652367.50.13152640.8564081.91.77.6965223.90.124405
237.75753268.60.19672351.87044.23.87.757531.75.40.195657
248.1559076.10.0590792242.22825.14.68.15577.7240.0523814
258.65705910.226522249.3520421.219.18.65707.9240.219851
268.89721930.25976940.3854990.90.88.89720.54.30.0508534
279.08573494.70.269819124.195769.58.69.085737.812.10.196181
PeakCounter: 27
DISPLAY GEL LANE LETTER: Lane e
DIGITAL REPORT GEL LANE: 6
LANE TYPE: Specimen
ACTUAL GEL LANE: 6
Go to Gel Schematic
Return to Lane Navigator
BandpILoc% RFAmplitudeWidthIntegr. OD% Integr. OD% Lane IntODpIG. LocG. SigmaG. Ampl
14.646536.80.023919160.2507910.50.44.646546.30.0223915
24.8989111.80.053978370.5609391.114.89890.26.50.0485694
34.99110513.60.11507881.496812.92.64.991105.67.60.111253
45.39817122.10.074073780.9069991.71.65.398172.57.80.0655961
55.55419825.60.15701792.570914.94.55.554195.59.10.158738
65.83624731.90.017398340.1225120.20.25.836246.34.10.0169777
76.10729538.10.25819931.520842.92.76.107294.93.40.255458
86.18630939.90.16786151.411862.72.56.1863095.40.147285
96.27132441.90.051271970.7408711.41.36.271322.27.10.0588306
106.37834344.30.5436333.262996.35.76.3783433.40.539067
BandpILoc% RFAmplitudeWidthIntegr. OD% Integr. OD% Lane IntODpIG. LocG. SigmaG. Ampl
116.48536246.80.31262542.169124.23.86.485361.240.305525
126.56237548.40.14689951.398742.72.46.562373.35.30.14894
136.67438950.30.32869931.911133.73.36.674389.13.40.315927
146.722395510.44436932.518194.84.46.722395.23.30.428704
156.8140652.50.1544141.0563221.86.81406.23.80.155839
167.159449580.23674131.41892.72.57.159448.73.40.232908
177.346472610.28056242.098543.77.346471.64.30.275817
187.40648162.10.37884542.407194.64.27.406481.53.70.371219
197.51949864.30.41651843.148776.15.57.519497.74.40.407351
207.73753168.60.22259931.090542.11.97.737530.62.80.216298
BandpILoc% RFAmplitudeWidthIntegr. OD% Integr. OD% Lane IntODpIG. LocG. SigmaG. Ampl
217.79654069.80.30148752.521664.94.47.7965404.80.29774
228.71670991.60.2816522311.138821.419.58.716709.722.90.274824
238.92272393.40.30454350.49958410.98.922722.94.60.0619019
249.23174496.10.276737115.7189311109.231738.511.30.28528
PeakCounter: 24
DISPLAY GEL LANE LETTER: Lane f
DIGITAL REPORT GEL LANE: 7
LANE TYPE: pI Markers
ACTUAL GEL LANE: 7
Go to Gel Schematic
Return to Lane Navigator
BandpILoc% RFAmplitudeWidthIntegr. OD% Integr. OD% Lane IntODpIG. LocG. SigmaG. Ampl
14.55344.40.084012660.7780227.96.44.5533.65.60.0778372
25.213617.60.1315951.1374111.69.45.2136.450.128465
35.8525032.30.16258141.2318712.510.15.85249.84.40.158344
46.5537548.40.10964981.475261512.16.55374.17.90.104797
56.8541153.10.026276170.2824312.92.36.85411.66.60.0242561
67.3547461.20.066877860.7142087.35.97.35474.56.30.0635541
78.1559877.30.098963371.237112.610.28.15597.37.20.0964993
88.4566285.50.070631791.0648610.88.88.45661.18.50.070434
98.6570591.10.064726650.5785425.94.88.65704.75.20.0624093
109.375096.90.11301471.3236913.510.99.3749.67.30.102218
PeakCounter: 10
Gel Banding Patterns Display in HTML using GIF stacking technique
(Uses Migration Position Normalized Data from above text peak reports)

Return to Lane Navigator    Go to Gel Schematic Key

a b c d e f
MK SP MK CO SP MK
white spacer for gel schematic
pI 4.55 label for gel schematic
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pI 5.20 label for gel schematic
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pI 5.8 label for gel schematic
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pI 6.55 label for gel schematic
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pI 4.55 label for gel schematic
white spacer for gel schematic
pI 5.20 label for gel schematic
white spacer for gel schematic
pI 5.8 label for gel schematic
white spacer for gel schematic
pI 6.55 label for gel schematic
white spacer for gel schematic
pI 6.85 label for gel schematic
white spacer for gel schematic
pI 7.35 label for gel schematic
white spacer for gel schematic
pI 8.15 label for gel schematic
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pI 8.45 label for gel schematic
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Key to Gel Schematic: MK=pI Marker Lanes, pI bands are in red
SP=Specimen Lanes, Specimen bands are in blue
CO=Co-migrated Lanes of pI Markers and Specimen (pI bands are in red, Specimen bands are in blue)
Note:  In co-migrated lanes, some pI Markers and Specimen bands may overlap in experiment.  Red pI
band is reported when overlapped peak position is equal to nominal pI Marker position.  Blue Specimen
band is reported when overlapped peak position deviates slightly from nominal pI Marker position.
Weak (<2% rel. intensity), Medium (2-6% rel. intensity), and Strong (>6% rel. intensity) band intensities indicated by gel schematic band thickness.
Return to Top of Gel Schematic

PeakCount: 106
HTML Forms TextArea Normalized Peak-Position Peak-Area for Copy-Paste events
DISPLAY GEL LANE LETTER: Lane a
DIGITAL REPORT GEL LANE: 2
LANE TYPE: pI Markers
ACTUAL GEL LANE: 2
PeakCount: 10 Return to Lane Navigator
DISPLAY GEL LANE LETTER: Lane b
DIGITAL REPORT GEL LANE: 3
LANE TYPE: Specimen
ACTUAL GEL LANE: 3
PeakCount: 25 Return to Lane Navigator
DISPLAY GEL LANE LETTER: Lane c
DIGITAL REPORT GEL LANE: 4
LANE TYPE: pI Markers
ACTUAL GEL LANE: 4
PeakCount: 10 Return to Lane Navigator
DISPLAY GEL LANE LETTER: Lane d
DIGITAL REPORT GEL LANE: 5
LANE TYPE: Co-Migrated
ACTUAL GEL LANE: 5
PeakCount: 27 Return to Lane Navigator
DISPLAY GEL LANE LETTER: Lane e
DIGITAL REPORT GEL LANE: 6
LANE TYPE: Specimen
ACTUAL GEL LANE: 6
PeakCount: 24 Return to Lane Navigator
DISPLAY GEL LANE LETTER: Lane f
DIGITAL REPORT GEL LANE: 7
LANE TYPE: pI Markers
ACTUAL GEL LANE: 7
PeakCount: 10 Return to Lane Navigator



 RETURN TO TOP THIS DOCUMENT
EXPERIMENTAL CONDITIONS: Gels were scanned at 600 d.p.i. in Adobe Photoshop 3.02 using a Sharp JX-325 Scanner with Film Scanner Attachment. Gels were analyzed for Densitometry, Intregration, and Migration Position Normalization in EXCEL 5.0 Format. *.xls files were imported into Paradox 7.0 to save as *.db files.

 RETURN TO TOP THIS DOCUMENT
Delphi 4.0 Generation of this Isoelectric Focusing (IEF) RFE PAGE 5 Tools in HTML
This HTML is automatically generated from a series of Paradox 7.0 database filesof IEF peak reports is controlled with a main RFEIEF Paradox 7.0 database withDelphi 3.0 front end (see Delphi 2.0 form) and back end for facile addition and modification of the Content of these RFE PAGE 5 HTML tools.
Contact Brad Tenge at btenge@ora.fda.gov for details.


RFE Contacts
A summary list of RFE contact personnel is available at http://www.cfsan.fda.gov/~frf/rfecon.html.

For isoelectric focusing laboratory experiments for fish species identification, you may contact

For isoelectric focusing gel analysis and pattern recognition applications, you may contact

For CFSAN web consultation and posting, you may contact


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Last updated on 2003-FEB-27 by bjt/frf/nln